Anticorps Recombinant de lapin anti-FOXP4

FOXP4 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

242356B4

N° de cat : 84855-1-RR

Synonymes

Fork head-related protein-like A, FKHLA, 242356B4

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Galerie de données de validation

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  IF Staining of HeLa using 84855-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FOXP4 antibody (84855-1-RR, Clone: 242356B4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 84855-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FOXP4 antibody (84855-1-RR, Clone: 242356B4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 84855-1-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FOXP4 Recombinant antibody (84855-1-RR, Clone:242356B4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 84855-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84855-1-RR against Human FOXP4 were performed. The affinity constant is 0.402 nM.

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Applications testées

• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84855-1-RR cible FOXP4 dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FOXP4 Protéine recombinante Ag10047
• Nom complet: forkhead box P4
• Masse moléculaire calculée: 678 aa, 73 kDa
• Numéro d’acquisition GenBank: BC052803
• Symbole du gène: FOXP4
• Identification du gène (NCBI): 116113
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FOXP4, also named as FKHLA, belongs to the FOX family. FOXP4 has been implicated in diverse biological processes in tumor initiation and progression. It has been demonstrated that FOXP4 is significantly upregulated in breast cancer, hepatocellular carcinoma and oral squamous cell carcinoma (PMID: 34590150). FOXP4 has 3 isoforms with the molecular mass of 72-73 kDa.

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