Anticorps Monoclonal anti-FRS2

FRS2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG3

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4E10H12

N° de cat : 66263-1-PBS

Synonymes

SNT, FRS2alpha, FRS2A, FGFR-signaling adaptor SNT, FGFR substrate 2

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Galerie de données de validation

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  WB analysis of MCF-7 using 66263-1-Ig (same clone as 66263-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66263-1-Ig (FRS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66263-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66263-1-Ig (same clone as 66263-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66263-1-Ig (FRS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66263-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66263-1-Ig (same clone as 66263-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with (FRS2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66263-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66263-1-Ig (same clone as 66263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66263-1-Ig (FRS2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66263-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66263-1-Ig (same clone as 66263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66263-1-Ig (FRS2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66263-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66263-1-Ig (same clone as 66263-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FRS2 antibody (66263-1-Ig, Clone: 4E10H12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66263-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66263-1-PBS cible FRS2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG3
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FRS2 Protéine recombinante Ag19299
• Nom complet: fibroblast growth factor receptor substrate 2
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC021562
• Symbole du gène: FRS2
• Identification du gène (NCBI): 10818
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) has an alternative name as SNT-1, it is an adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. FGF receptor substrates (FRS2 and FRS3) are key adaptor proteins that mediate FGF-FGFR signalling in benign as well as malignant tissue. FRS2 is a 508 amino-acid protein,which is phosphorylated on tyrosine residues. The molecular weight of non-phosphorylated FRS2 is 57-68 kDa, but phosphorylated FRS2 is 80-90 kDa. Phosphorylation of FRS2 is associated with activation of a number of MAP kinases. Allele-specific regulation of FGFR2 mRNA expression with a mildly increased breast cancer risk has been reported.

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