Anticorps Polyclonal de lapin anti-FRY

FRY Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 32075-1-PBS

Synonymes

C13orf14, FRY Microtubule Binding Protein, Protein furry homolog

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Galerie de données de validation

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  WB analysis using 32075-1-AP (same clone as 32075-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 32075-1-AP (FRY antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32075-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 32075-1-AP (same clone as 32075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 32075-1-AP (FRY antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32075-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 32075-1-AP (same clone as 32075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 32075-1-AP (FRY antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32075-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 32075-1-AP (same clone as 32075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 32075-1-AP (FRY antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32075-1-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 32075-1-AP (same clone as 32075-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using FRY antibody (32075-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 32075-1-PBS in a different storage buffer formulation.

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Informations sur le produit

32075-1-PBS cible FRY dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FRY Protéine recombinante Ag34959
• Nom complet: furry homolog (Drosophila)
• Masse moléculaire calculée: 339 kDa
• Poids moléculaire observé: 340 kDa
• Numéro d’acquisition GenBank: NM_023037
• Symbole du gène: FRY
• Identification du gène (NCBI): 10129
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The FRY gene encodes a protein involved in cell cycle regulation and DNA damage response. It plays a crucial role in the G2/M checkpoint, ensuring cells complete DNA repair before entering mitosis by activating the ATR-CHK1 signaling pathway. FRY is expressed in various tissues, including brain, liver, and testis, with notable expression in Sertoli and spermatogonial cells. Aberrant FRY function is associated with multiple diseases, such as cancer and neurodegenerative disorders. In cancer cells, FRY expression can influence proliferation and survival. Understanding FRY's role in cell cycle control and genome stability is vital for elucidating disease mechanisms and developing targeted therapies.

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