Anticorps Polyclonal de lapin anti-FSHR

• Phare
• Validé par KD/KO

FSHR Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (4)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

72

Avis (1)

N° de cat : 22665-1-AP

Synonymes

Follitropin receptor, FSH R, FSHR, FSHRO, LGR1, ODG1

---

Galerie de données de validation

• 

  WB analysis using 22665-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 22665-1-AP (FSHR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat ovary using 22665-1-AP

  rat ovary tissue were subjected to SDS PAGE followed by western blot with 22665-1-AP (FSHR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 22665-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 22665-1-AP (FSHR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 22665-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22665-1-AP (FSHR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22665-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22665-1-AP (FSHR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse ovary using 22665-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22665-1-AP (FSHR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 22665-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22665-1-AP (FSHR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 22665-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22665-1-AP (FSHR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 22665-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FSHR antibody (22665-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 22665-1-AP

  1X10^6 HeLa cells were stained with .2ug FSHR antibody (22665-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Applications testées

• Résultats positifs en WB: tissu ovarien de souris, cellules DU 145, cellules HeLa, cellules PC-3, tissu ovarien de rat
• Résultats positifs en IHC: tissu ovarien de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules HepG2
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 34 publications below
• IHC: See 12 publications below
• IF: See 40 publications below
• FC: See 3 publications below

Informations sur le produit

22665-1-AP cible FSHR dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Chèvre, Humain, poulet, souris, Oie
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FSHR Protéine recombinante Ag18158
• Nom complet: follicle stimulating hormone receptor
• Masse moléculaire calculée: 695 aa, 78 kDa
• Poids moléculaire observé: 71-78 kDa
• Numéro d’acquisition GenBank: BC118548
• Symbole du gène: FSHR
• Identification du gène (NCBI): 2492
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	Cell Res; Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol.; Authors - Yanjing Guo
human,mouse	WB	Autophagy; Autophagy regulates differentiation of ovarian granulosa cells through degradation of WT1; Authors - Tong Shao
mouse	IHC	Redox Biol; Novel ovarian endometriosis model causes infertility via iron-mediated oxidative stress in mice.; Authors - Shotaro Hayashi
human	IF	ACS Appl Mater Interfaces; A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells; Authors - Xinyang Zhao
human,mouse	WB,IHC	Free Radic Biol Med; Iron overload modulates follicular microenvironment via ROS/HIF-1α/FSHR signaling; Authors - Yaoqiu Wu
mouse	IF	Ecotoxicol Environ Saf; Chlorogenic acid rescues zearalenone induced injury to mouse ovarian granulosa cells.; Authors - YanYan Yi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Nolan (Verified Customer) (04-11-2022)

Antibody works better if you stain at room temperature for 2 hours instead of 4C overnight.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse FSHR

Produits associés

LHCGR Polyclonal antibody

19968-1-AP

androgen receptor Polyclonal antibody

22576-1-AP

CYP19A1 Polyclonal antibody

16554-1-AP

APPL1 Polyclonal antibody

12639-1-AP