Anticorps Monoclonal anti-Follistatin

• Phare
• Validé par KD/KO

Follistatin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(8)

CloneNo.

4E9B10

Avis (1)

N° de cat : 60060-1-Ig

Synonymes

FST, FS, Activin-binding protein, Activin binding protein, 4E9B10

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Galerie de données de validation

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  WB analysis using 60060-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60060-1-Ig (Follistatin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 60060-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 60060-1-Ig (Follistatin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 60060-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60060-1-Ig (Follistatin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 60060-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60060-1-Ig (Follistatin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 60060-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60060-1-Ig (Follistatin antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 60060-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60060-1-Ig (Follistatin antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 60060-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Follistatin antibody (60060-1-Ig, Clone: 4E9B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HepG2, cellules NCI-H1299, cellules Raji, cellules SKOV-3, cellules U2OS
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Informations sur le produit

60060-1-Ig cible Follistatin dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Follistatin Protéine recombinante Ag0775
• Nom complet: follistatin
• Masse moléculaire calculée: 33 aa, 7 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC004107
• Symbole du gène: Follistatin
• Identification du gène (NCBI): 10468
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Follistatin (FST) is a member of the tissue growth factor β family and is a secreted glycoprotein that antagonizes many members of the family, including activin A, growth differentiation factor11 , and myostatin. It binds activin A with high affinity and whose expression can be induced by activin A and several other pro-inflammatory cytokines. Activin A-follistatin complexes are biologically inactive and bind to cell surface heparan sulphate-containing proteoglycans for internalisation and degradation. It has also been found to play a significant role in the management of skeletal muscle size and mass. It also has important roles in early embryonic development, differentiation of ovarian granulosa cells, liver fibrosis and polycystic ovarian syndrome. FS 288 glycosylation results in a major species of 35 kDa and a less abundant 40 kDa and minor products of 40 and 42 kDa (PMID: 9785474). Three typical follistatin bands are at 32, 35, and 39 kDa (PMID: 2036994).

Publications

Species	Application	Title
rat	IHC	Cells; Tubule-Derived Follistatin Is Increased in the Urine of Rats with Renal Ischemia and Reflects the Severity of Acute Tubular Damage; Authors - Izumi Nagayama
mouse	IHC	Antioxid Redox Signal; Follistatin Protects against Glomerular Mesangial Cell Apoptosis and Oxidative Stress to Ameliorate Chronic Kidney Disease.; Authors - Neel Mehta
mouse	WB	Front Pharmacol; Follistatin Attenuates Myocardial Fibrosis in Diabetic Cardiomyopathy via the TGF-β-Smad3 Pathway.; Authors - Yinhui Wang
human	IF	Fertil Steril; Myostatin, follistatin and activin type II receptors are highly expressed in adenomyosis.; Authors - Patrizia Carrarelli
human,mouse	WB,IF	PeerJ; Development of a gene doping detection method to detect overexpressed human follistatin using an adenovirus vector in mice.; Authors - Koki Yanazawa
camel	WB,IF	Animals (Basel); Screening and Identification of Differential Ovarian Proteins before and after Induced Ovulation via Seminal Plasma in Bactrian Camels.; Authors - Qi Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jennifer (Verified Customer) (07-17-2023)

I am not sure what this antibody targets, but I am sure it is not Follistatin. This is a glycosylated protein that appears as a smear at ~40 - 50 kDa. However, with this antibody there is a single band at ~ 35 kDa. We have validated several FST antibodies using knock-down and overexpression cell lines so we know what to expect.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5,000
• Cell Tissue Type: HNSCC cell lines

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