Anticorps Polyclonal de lapin anti-FTCD

FTCD Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

Avis (1)

N° de cat : 21959-1-AP

Synonymes

Glutamate formyltransferase, Glutamate formiminotransferase, Glutamate formimidoyltransferase, Formiminotransferase-cyclodeaminase, Formimidoyltransferase-cyclodeaminase

---

Galerie de données de validation

• 

  WB analysis using 21959-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21959-1-AP (FTCD antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 21959-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21959-1-AP (FTCD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 21959-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21959-1-AP (FTCD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique de rat
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:4000-1:16000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

21959-1-AP cible FTCD dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FTCD Protéine recombinante Ag16646
• Nom complet: formiminotransferase cyclodeaminase
• Masse moléculaire calculée: 541 aa, 59 kDa
• Poids moléculaire observé: 53-62 kDa
• Numéro d’acquisition GenBank: BC136395
• Symbole du gène: FTCD
• Identification du gène (NCBI): 10841
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FTCD (formimidoyltransferase cyclodeaminase) is critical for the catabolism of histidine, a . This process is one-carbon units that can enter the one-carbon/folate cycle, which provides methyl groups for arsenic metabolism (PMID: 30893314). In addition, FTCD could serve as a predictive and prognostic marker for patients with HCC (PMID: 37675273).

Publications

Species	Application	Title
human	WB	Sci Rep; Quantitative proteomics by SWATH-MS reveals sophisticated metabolic reprogramming in hepatocellular carcinoma tissues.; Authors - Yanyan Gao
human	IF	Front Cell Dev Biol; Significance of Liver Zonation in Hepatocellular Carcinoma.; Authors - Shizhe Yu
human	IHC	Bioengineered; A novel four-gene of iron metabolism-related and methylated for prognosis prediction of hepatocellular carcinoma.; Authors - Huimin Shen
human	WB,IHC	Med Sci Monit; Formiminotransferase Cyclodeaminase Suppresses Hepatocellular Carcinoma by Modulating Cell Apoptosis, DNA Damage, and Phosphatidylinositol 3-Kinases (PI3K)/Akt Signaling Pathway.; Authors - Jiajia Chen
mouse	WB	FASEB J; Dysregulation of hepatic one-carbon metabolism in classical homocystinuria: Implications of redox-sensitive DHFR repression and tetrahydrofolate depletion for pathogenesis and treatment; Authors - Kenneth N Maclean
Human		iScience; Crosstalk of non-apoptotic RCD panel in hepatocellular carcinoma reveals the prognostic and therapeutic optimization; Authors - Shuo Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Hua (Verified Customer) (05-11-2022)

Very good antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: House liver/HepG2

FTCD Antibody Western Blot validation (1:4000 dilution) in House liver/HepG2 (Cat no:21959-1-AP)

{{ptg:RelatedPrimaryAntibodies}}