Anticorps Monoclonal anti-FTCD

• Phare
• Validé par KD/KO

FTCD Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1H8A1

N° de cat : 66979-1-Ig

Synonymes

Formimidoyltetrahydrofolate cyclodeaminase, Formimidoyltransferase-cyclodeaminase, Formiminotransferase-cyclodeaminase, Glutamate formimidoyltransferase, Glutamate formiminotransferase

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Galerie de données de validation

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  WB analysis of mouse liver using 66979-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66979-1-Ig (FTCD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig kidney using 66979-1-Ig

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 66979-1-Ig (FTCD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 66979-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66979-1-Ig (FTCD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66979-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66979-1-Ig (FTCD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 66979-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66979-1-Ig (FTCD antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 66979-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66979-1-Ig (FTCD antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66979-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66979-1-Ig (FTCD antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66979-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66979-1-Ig (FTCD antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 66979-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FTCD antibody (66979-1-Ig, Clone: 1H8A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver cancer using 66979-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FTCD antibody (66979-1-Ig, Clone: 1H8A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse kidney using 66979-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using FTCD antibody (66979-1-Ig, Clone: 1H8A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique de porc, tissu hépatique de rat, tissu rénal de porc
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du foie humain, tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:3000-1:10000
• Immunohistochimie (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

66979-1-Ig cible FTCD dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FTCD Protéine recombinante Ag17429
• Nom complet: formiminotransferase cyclodeaminase
• Masse moléculaire calculée: 541 aa, 59 kDa
• Poids moléculaire observé: 53-62 kDa
• Numéro d’acquisition GenBank: BC136395
• Symbole du gène: FTCD
• Identification du gène (NCBI): 10841
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FTCD (formimidoyltransferase cyclodeaminase) is critical for the catabolism of histidine. This process is one-carbon units that can enter the one-carbon/folate cycle, which provides methyl groups for arsenic metabolism (PMID: 30893314). In addition, FTCD could serve as a predictive and prognostic marker for patients with HCC (PMID: 37675273).

Publications

Species	Application	Title
mouse	WB	Biomolecules; Exosome-Related FTCD Facilitates M1 Macrophage Polarization and Impacts the Prognosis of Hepatocellular Carcinoma; Authors - Youyi Liu ; KD Validated

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