Anticorps Polyclonal de lapin anti-Ferritin heavy chain

• Phare
• Validé par KD/KO

Ferritin heavy chain Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(38)

N° de cat : 11682-1-AP

Synonymes

Ferritin, FTH1, Cell proliferation-inducing gene 15 protein, EC:1.16.3.1, Ferritin heavy chain, N-terminally processed

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Galerie de données de validation

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  WB analysis of HeLa using 11682-1-AP

  WB result of Ferritin heavy chain antibody (11682-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ferritin heavy chain transfected HeLa cells.

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  WB analysis using 11682-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11682-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11682-1-AP

  IP result of anti-Ferritin heavy chain (IP:11682-1-AP, 4ug; Detection:11682-1-AP 1:3000) with HeLa cells lysate 1600 ug.

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  IHC staining of rat liver using 11682-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 11682-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 11682-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 11682-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11682-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ferritin heavy chain antibody (11682-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 11682-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ferritin heavy chain antibody (11682-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules L02, tissu hépatique de rat, tissu hépatique de souris, tissu placentaire humain
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu hépatique de souris, tissu hépatique de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 34 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

11682-1-AP cible Ferritin heavy chain dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Ferritin heavy chain Protéine recombinante Ag2290
• Nom complet: ferritin, heavy polypeptide 1
• Masse moléculaire calculée: 183 aa, 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC000857
• Symbole du gène: FTH1
• Identification du gène (NCBI): 2495
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.

Publications

Species	Application	Title
human	WB	Free Radic Biol Med; S100A8 induces Cyclophosphamide-Induced Alopecia via NCF2/NOX2-Mediated Ferroptosis; Authors - Wen Xu
mouse	WB	Cells; Unveiling Smyd-2's Role in Cytoplasmic Nrf-2 Sequestration and Ferroptosis Induction in Hippocampal Neurons After Cerebral Ischemia/Reperfusion; Authors - Daohang Liu
	WB	Inflammopharmacology; Sulfasalazine promotes ferroptosis through AKT-ERK1/2 and P53-SLC7A11 in rheumatoid arthritis; Authors - Chenyu Zhao
mouse	WB	Mol Neurobiol; A Disintegrin and Metalloproteinase-8 Protects Against Erastin-Induced Neuronal Ferroptosis via Activating Nrf2/HO-1/FTH1 Signaling Pathway; Authors - Zhanyang Qian
human	WB	BMC Cancer; Oridonin-induced ferroptosis and apoptosis: a dual approach to suppress the growth of osteosarcoma cells; Authors - Feifan Zhang
sheep	WB	J Dairy Sci; Short-term effects of Subacute ruminal acidosis on ferroptosis and iron metabolism in the livers of lactating sheep fed a high-grain diet; Authors - Hongzhu Zhang

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