Anticorps Monoclonal anti-Ferritin heavy chain

Ferritin heavy chain Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

3G8D7

N° de cat : 60875-5-Ig

Synonymes

Ferritin, FTH1, Cell proliferation-inducing gene 15 protein, EC:1.16.3.1, Ferritin heavy chain, N-terminally processed

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Galerie de données de validation

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  WB analysis using 60875-5-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60875-5-Ig (Ferritin heavy chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 60875-5-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60875-5-Ig (Ferritin heavy chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat liver using 60875-5-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60875-5-Ig (FTH1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 60875-5-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60875-5-Ig (FTH1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 60875-5-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ferritin heavy chain antibody (60875-5-Ig, Clone: 3G8D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of HepG2 using 60875-5-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ferritin heavy chain antibody (60875-5-Ig, Clone: 3G8D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules K-562, tissu cérébral de lapin, tissu cérébral de rat
• Résultats positifs en IHC: tissu hépatique de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

60875-5-Ig cible Ferritin heavy chain dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat

• Réactivité: Humain, Lapin, rat
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Ferritin heavy chain Protéine recombinante Ag21386
• Nom complet: ferritin, heavy polypeptide 1
• Masse moléculaire calculée: 183 aa, 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC000857
• Symbole du gène: FTH1
• Identification du gène (NCBI): 2495
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.

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