Anticorps Recombinant de lapin anti-FTO

• Phare
• Validé par KD/KO

FTO Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4L4

N° de cat : 81471-1-PBS

Synonymes

4L4, KIAA1752

---

Galerie de données de validation

• 

  WB analysis using 81471-1-RR (same clone as 81471-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81471-1-RR (FTO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81471-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MDA-MB-231 using 81471-1-RR (same clone as 81471-1-PBS)

  WB result of FTO antibody (81471-1-RR; 1:600; room temperature for 1.5 hours) with wild-type and FTO knockout MDA-MB-231 cells. This data was developed using the same antibody clone with 81471-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 81471-1-RR (same clone as 81471-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FTO antibody (81471-1-RR, Clone: 4L4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81471-1-PBS in a different storage buffer formulation.

• 

  FC experiment of NIH/3T3 using 81471-1-RR (same clone as 81471-1-PBS)

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug FTO Recombinant antibody (81471-1-RR, Clone:4L4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 81471-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

81471-1-PBS cible FTO dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FTO Protéine recombinante Ag26095
• Nom complet: fat mass and obesity associated
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: NM_001080432
• Symbole du gène: FTO
• Identification du gène (NCBI): 79068
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in th1e FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.

{{ptg:RelatedPrimaryAntibodies}}