Anticorps Polyclonal de lapin anti-FTSJD2

FTSJD2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 27707-1-AP

Synonymes

FtsJ methyltransferase domain-containing protein 2, EC:2.1.1.57, CMTR1, Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1, Cap1 2'O-ribose methyltransferase 1

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Galerie de données de validation

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  WB analysis using 27707-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27707-1-AP (FTSJD2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 27707-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27707-1-AP (FTSJD2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 27707-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27707-1-AP (FTSJD2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 27707-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27707-1-AP (FTSJD2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 27707-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27707-1-AP (FTSJD2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 27707-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FTSJD2 antibody (27707-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules U-251, cellules NIH/3T3
• Résultats positifs en IHC: tissu testiculaire humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

27707-1-AP cible FTSJD2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FTSJD2 Protéine recombinante Ag26722
• Nom complet: FtsJ methyltransferase domain containing 2
• Masse moléculaire calculée: 835 aa, 95 kDa
• Poids moléculaire observé: 96-100 kDa
• Numéro d’acquisition GenBank: BC031890
• Symbole du gène: FTSJD2
• Identification du gène (NCBI): 23070
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cap1 2′-O-methylation is catalyzed by cap methyltransferase 1 (CMTR1). CMTR1, also called IFN-stimulated gene 95 kDa protein (ISG95) or FTSJD2, is elevated by a viral infection and required to promote IFN-mediated induction of ISGs and the antiviral response. High CMTR1 expression is associated with poor prognosis in patients with CRC (PMID: 37024465). CMTr1 binds ATP-dependent RNA DHX15 helicase and that this interaction, mediated by the G-patch domain of CMTr1, has an advantageous effect on CMTr1 activity towards highly structured RNA substrates (PMID: 30397098).). Human CMTR1 is a 95 kDa nuclear protein that has multiple domains, consisting of a G-patch domain, a RrmJ/FtsJ methyltransferase domain, a non-functional cap guanylyltransferase-like domain and a WW domain (PMID: 30312682).

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