Anticorps Polyclonal de lapin anti-FUBP1

• Phare
• Validé par KD/KO

FUBP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(8)

Avis (1)

N° de cat : 24864-1-AP

Synonymes

FUSE-binding protein 1, Far upstream element-binding protein 1

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 24864-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24864-1-AP (FUBP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 24864-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24864-1-AP (FUBP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 24864-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24864-1-AP (FUBP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 24864-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24864-1-AP (FUBP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 24864-1-AP

  IP result of anti-FUBP1 (IP:24864-1-AP, 4ug; Detection:24864-1-AP 1:300) with SH-SY5Y cells lysate 800ug.

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  IF Staining of HeLa using 24864-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FUBP1 antibody (24864-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules Jurkat, cellules K-562, tissu cérébral de souris
• Résultats positifs en IP: cellules SH-SY5Y
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

24864-1-AP cible FUBP1 dans les applications de WB, IF/ICC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FUBP1 Protéine recombinante Ag13149
• Nom complet: far upstream element (FUSE) binding protein 1
• Masse moléculaire calculée: 653 aa, 69 kDa
• Poids moléculaire observé: 69 kDa
• Numéro d’acquisition GenBank: BC017247
• Symbole du gène: FUBP1
• Identification du gène (NCBI): 8880
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FUBP1 also termed as FBP and FUSE-binding protein 1 is 644 amino-acid protein, which localizes in the nucleus. FUBP1 binds to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter and regulates the MYC expression, indicating that FBP1 functions as a growth-dependent regulator of c-Myc expression. FUBP1 binds to FUSE, and PUF60, and forms a stable tripartite complex, which represses activated but not basal c-myc transcription after transcription initiation by delaying promoter escape. FUBP1 may be acting both as activator and repressor of transcription.

Publications

Species	Application	Title
human	WB	Int J Biol Sci; Poly(U) binding splicing factor 60 promotes renal cell carcinoma growth by transcriptionally upregulating telomerase reverse transcriptase.; Authors - Qian Long
human	WB	Biology (Basel); In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.; Authors - Olga Gourdomichali
human	WB	Anticancer Res; Comparative Proteomic Profiling of Cisplatin-resistant Nasopharyngeal Carcinoma Cell Lines: Novel Biomarkers for Improving Chemotherapy of NPC.; Authors - Parisa Prathaphan
human	WB	J Adv Res; LncRNA NIPA1-SO confers atherosclerotic protection by suppressing the transmembrane protein NIPA1; Authors - Min Jiang
human	ChIP	MedComm (2020); Far upstream element-binding protein 1 confers lobaplatin resistance by transcriptionally activating PTGES and facilitating the arachidonic acid metabolic pathway in osteosarcoma; Authors - Qiong Ma
human	WB	J Immunother Cancer; The NR_109/FUBP1/c-Myc axis regulates TAM polarization and remodels the tumor microenvironment to promote cancer development; Authors - Cong Zhang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tatyana (Verified Customer) (12-22-2024)

ICC with antibody diluted in 5% goat serum in PBST, with 2 h incubation at RT. Clean nuclear staining.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa/human

FUBP1 Antibody Immunofluorescence validation (1:1000 dilution) in HeLa/human (Cat no:24864-1-AP)

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