Anticorps Polyclonal de lapin anti-FUBP1

• Phare
• Validé par KD/KO

FUBP1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24864-1-PBS

Synonymes

FUSE-binding protein 1, Far upstream element-binding protein 1

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 24864-1-AP (same clone as 24864-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24864-1-AP (FUBP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24864-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 24864-1-AP (same clone as 24864-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 24864-1-AP (FUBP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24864-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 24864-1-AP (same clone as 24864-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24864-1-AP (FUBP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24864-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 24864-1-AP (same clone as 24864-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24864-1-AP (FUBP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24864-1-PBS in a different storage buffer formulation.

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  IP experiment of SH-SY5Y using 24864-1-AP (same clone as 24864-1-PBS)

  IP result of anti-FUBP1 (IP:24864-1-AP, 4ug; Detection:24864-1-AP 1:300) with SH-SY5Y cells lysate 800ug. This data was developed using the same antibody clone with 24864-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 24864-1-AP (same clone as 24864-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FUBP1 antibody (24864-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 24864-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24864-1-PBS cible FUBP1 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FUBP1 Protéine recombinante Ag13149
• Nom complet: far upstream element (FUSE) binding protein 1
• Masse moléculaire calculée: 653 aa, 69 kDa
• Poids moléculaire observé: 69 kDa
• Numéro d’acquisition GenBank: BC017247
• Symbole du gène: FUBP1
• Identification du gène (NCBI): 8880
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FUBP1 also termed as FBP and FUSE-binding protein 1 is 644 amino-acid protein, which localizes in the nucleus. FUBP1 binds to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter and regulates the MYC expression, indicating that FBP1 functions as a growth-dependent regulator of c-Myc expression. FUBP1 binds to FUSE, and PUF60, and forms a stable tripartite complex, which represses activated but not basal c-myc transcription after transcription initiation by delaying promoter escape. FUBP1 may be acting both as activator and repressor of transcription.

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