Anticorps Polyclonal de lapin anti-FUCA2

FUCA2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30080-1-PBS

Synonymes

Alpha-L-fucosidase 2, Alpha-L-fucoside fucohydrolase 2, EC:3.2.1.51, Plasma alpha-L-fucosidase, PSEC0151

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Galerie de données de validation

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  WB analysis using 30080-1-AP (same clone as 30080-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 30080-1-AP (FUCA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30080-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 30080-1-AP (same clone as 30080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30080-1-AP (FUCA2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30080-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 30080-1-AP (same clone as 30080-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FUCA2 antibody (30080-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 30080-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30080-1-PBS cible FUCA2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FUCA2 Protéine recombinante Ag32467
• Nom complet: fucosidase, alpha-L- 2, plasma
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 54-60 kDa
• Numéro d’acquisition GenBank: BC003060
• Symbole du gène: FUCA2
• Identification du gène (NCBI): 2519
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Alpha-L-fucosidase 2 (FUCA2) is a gene that codes for a secreted non-lysosomal enzyme and belongs to the glycosyl hydrolase 29 (GH29) family. The enzyme is responsible for the removal of the alpha-1,6-fucose linked to N-acetylglucosamine residue of glycoproteins. Serum FUCA2 is a potential biomarker used for early detection of esophageal squamous cell carcinoma (ESCC) and hepatocellular carcinoma (HCC). FUCA2 has 2 isoforms with the molecular mass of 20 and 54 kDa (PMID: 34935631). Sometimes higher molecular weight around 60 kDa can also be observed, which may be a modified variant of FUCA2.

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