Anticorps Polyclonal de lapin anti-FUNDC2

FUNDC2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 19832-1-AP

Synonymes

HCC 3, HCBP6, FUN14 domain-containing protein 2, FUN14 domain containing 2, DC44

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Galerie de données de validation

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  WB analysis using 19832-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19832-1-AP (FUNDC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HuH-7 using 19832-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 19832-1-AP (FUNDC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 19832-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19832-1-AP (FUNDC2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 19832-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19832-1-AP (FUNDC2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules HuH-7
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

19832-1-AP cible FUNDC2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FUNDC2 Protéine recombinante Ag13800
• Nom complet: FUN14 domain containing 2
• Masse moléculaire calculée: 189 aa, 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC000255
• Symbole du gène: FUNDC2
• Identification du gène (NCBI): 65991
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FUNDC2, or FUN14 domain-containing protein 2, is a mitochondrial outer membrane protein that plays a significant role in various cellular processes, including the regulation of cell survival, apoptosis, and metabolism. It has been implicated in the progression of several types of cancer, particularly in triple-negative breast cancer (TNBC) and liver cancer. In the context of cancer, FUNDC2 has been shown to promote tumorigenesis. It is associated with poor prognosis in hepatocellular carcinoma (HCC) and is involved in mitochondrial fragmentation by inhibiting MFN1-mediated mitochondrial fusion, which can lead to changes in cellular metabolism and energy levels. FUNDC2 also plays a role in platelet function and survival. It binds directly to phosphatidylinositol-3,4,5-trisphosphate (PIP3), which is a key lipid second messenger in cell signaling, and this interaction is crucial for the recruitment of PIP3 to mitochondria. FUNDC2 deficiency has been shown to decrease platelet aggregation and impair hemostasis in mice, highlighting its importance in platelet function.

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