Anticorps Monoclonal anti-FURIN

FURIN Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D1B9

N° de cat : 67481-1-PBS

Synonymes

Dibasic processing enzyme, FUR, FURIN, PACE, PCSK3, SPC1

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Galerie de données de validation

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  WB analysis using 67481-1-Ig (same clone as 67481-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67481-1-Ig (FURIN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67481-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 67481-1-Ig (same clone as 67481-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67481-1-Ig (FURIN antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67481-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67481-1-Ig (same clone as 67481-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67481-1-Ig (FURIN antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67481-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67481-1-Ig (same clone as 67481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67481-1-Ig (FURIN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67481-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67481-1-Ig (same clone as 67481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67481-1-Ig (FURIN antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67481-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67481-1-PBS cible FURIN dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FURIN Protéine recombinante Ag13313
• Nom complet: furin (paired basic amino acid cleaving enzyme)
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 90-110 kDa
• Numéro d’acquisition GenBank: BC012181
• Symbole du gène: FURIN
• Identification du gène (NCBI): 5045
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FURIN, also named as FUR, PACE, PCSK3, SPC1, Kex2p, is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. Glycosylation and other post-translational modifications can make the pre-pro furin run at 100-110 kDa, the mature furin at 98-95 kDa and shed furin at 90 kDa.

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