Anticorps Polyclonal de lapin anti-FUS/TLS

• Phare
• Validé par KD/KO

FUS/TLS Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, chIP, ELISA

Conjugaison

Non conjugué

Publications

112

Avis (8)

N° de cat : 11570-1-AP

Synonymes

75 kDa DNA pairing protein, FUS, FUS1, FUS-CHOP, hnRNP P2, Oncogene FUS, Oncogene TLS, POMp75, RNA binding protein FUS, TLS, TLS/CHOP

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Galerie de données de validation

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  WB analysis of HEK-293 using 11570-1-AP

  WB result of FUS antibody (11570-1-AP, 1:5000) with si-Control and si-FUS transfected HEK 293 cells.

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  WB analysis of HeLa using 11570-1-AP

  HeLa (WT and FUS KO) lysates prepared with RIPA buffer, 30 μg protein loaded. 11570-1-AP incubated at 1:5000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• 

  WB analysis using 11570-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11570-1-AP (FUS/TLS antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 11570-1-AP

  IP result of anti-FUS/TLS(IP:11570-1-AP, 4ug; Detection:11570-1-AP 1:2000) with K-562 cells lysate 1760 ug.

• 

  IHC staining of mouse brain using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11570-1-AP (FUS/TLS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11570-1-AP (FUS/TLS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11570-1-AP (FUS/TLS antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11570-1-AP (FUS/TLS antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11570-1-AP (FUS/TLS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11570-1-AP (FUS/TLS Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human ovary tumor using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11570-1-AP (FUS/TLS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human ovary tumor using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11570-1-AP (FUS/TLS Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 11570-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 11570-1-AP (FUS/TLS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 11570-1-AP

  HeLa WT cells (yellow outline) and FUS KO cells (red outline) labelled with a green or a far-red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 11570-1-AP at 1:2000 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• 

  IF Staining of mouse colon using 11570-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using FUS/TLS antibody (11570-1-AP) at dilution of 1:200, CoraLite®594 smooth muscle actin antibody (CL594-14395, red), CD45 antibody (80297-1-RR, Clone: 6O19, orange), E-cadherin antibody (20874-1-AP, Magenta).

• 

  IF Staining of HeLa using 11570-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FUS/TLS antibody (11570-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 11570-1-AP

  1X10^6 HeLa cells were stained with 0.2ug FUS/TLS antibody (11570-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat, tissu de cancer du sein humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de côlon de souris, cellules HeLa, cellules HepG2
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 10 publications below
• WB: See 58 publications below
• IHC: See 32 publications below
• IF: See 46 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below
• RIP: See 4 publications below

Informations sur le produit

11570-1-AP cible FUS/TLS dans les applications de WB, RIP, IP, IHC, IF, FC, CoIP, chIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FUS/TLS Protéine recombinante Ag2150
• Nom complet: fusion (involved in t(12;16) in malignant liposarcoma)
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 68-75 kDa
• Numéro d’acquisition GenBank: BC026062
• Symbole du gène: FUS
• Identification du gène (NCBI): 2521
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)[PMID: 22640227]. This antibody is a rabbit polyclonal antibody raised against an internal region of human FUS. FUS was detected double bands of 68-74 kDa (PMID:31519807).

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	IHC,IF	Nature; Mutations in UBQLN2 cause dominant X-linked juvenile and adult-onset ALS and ALS/dementia.; Authors - Deng Han-Xiang HX
human,mouse	WB	Nat Med; Antisense oligonucleotide silencing of FUS expression as a therapeutic approach in amyotrophic lateral sclerosis.; Authors - Vladislav A Korobeynikov
human	WB,IF	Cell; Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains.; Authors - Lin Guo
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
mouse	WB	Nat Neurosci; FUS-mediated regulation of acetylcholine receptor transcription at neuromuscular junctions is compromised in amyotrophic lateral sclerosis.; Authors - Gina Picchiarelli ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xhuljana (Verified Customer) (03-01-2024)

Used in siRNA transfected C2C12 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2C12 cells

FUS/TLS Antibody Western Blot validation (1:1000 dilution) in C2C12 cells (Cat no:11570-1-AP)

FH

Zhongwen (Verified Customer) (09-25-2023)

I can find two bands in the target region. I am not sure which one is the target band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FUS/TLS Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:11570-1-AP)

FH

manohar (Verified Customer) (07-10-2023)

Nitrocellulose membrane is used with 5% milk as blocking and antibody diluted in 1% milk and incubated overnight.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293, Neuronal stem cells, Mouse tissue

FH

Tatyana (Verified Customer) (05-14-2023)

ICC using 5% goat serum/PBST buffer, 2 hours at RT. Good specific nuclear signal.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

FUS/TLS Antibody Immunofluorescence validation (1:1000 dilution) in HeLa (Cat no:11570-1-AP)

FH

shashirekha (Verified Customer) (12-23-2020)

Used for immunopreciptation at 1:1000 dilution. Works very well

• Applications: Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Rat cortical neurons

FUS/TLS Antibody Immunoprecipitation validation (1:1000 dilution) in Rat cortical neurons (Cat no:11570-1-AP)

FH

H (Verified Customer) (04-06-2020)

The antibody worked well for HCT116 cell line. Nuclear cytosolic fractionation clearly showed that FUS is dominantly in nucleus, and sub-fraction is present in cytosol.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HCT116

FUS/TLS Antibody Western Blot validation (1:2000 dilution) in HCT116 (Cat no:11570-1-AP)

FH

Karthik (Verified Customer) (04-24-2019)

Magenta- FUSBlue- MAP2FUS staining consistent obtained with this antibody is consistent with literature

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:250 overnight at 4 degrees
• Cell Tissue Type: motor neurons

FH

Yen-Chen (Verified Customer) (12-03-2018)

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: human motor neuron

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