Anticorps Monoclonal anti-FUS/TLS

• Phare
• Validé par KD/KO

FUS/TLS Monoclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

1B4F8

Avis (1)

N° de cat : 68262-1-Ig

Synonymes

FUS, 1B4F8, 75 kDa DNA pairing protein, 75 kDa DNA-pairing protein, ALS

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Galerie de données de validation

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  WB analysis using 68262-1-Ig

  Untreated and Calyculin A treated HeLa and HEK-293 cells were subjected to SDS PAGE followed by western blot with 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 68262-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 68262-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68262-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 68262-1-Ig

  WB result of FUS/TLS antibody (68262-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FUS/TLS transfected HepG2 cells.

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  WB analysis of HeLa using 68262-1-Ig

  WB result of FUS/TLS antibody (68262-1-Ig; 1:10000; room temperature for 1.5 hours) with negative control and FUS/TLS knockout HeLa cells.

• 

  IP experiment of K-562 using 68262-1-Ig

  IP result of anti-FUS/TLS (IP:68262-1-Ig, 4ug; Detection:68262-1-Ig 1:5000) with K-562 cells lysate 1800 ug.

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  IHC staining of rat brain using 68262-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68262-1-Ig (FUS/TLS antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 68262-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 68262-1-Ig (FUS/TLS antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 68262-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68262-1-Ig (FUS/TLS antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 68262-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 68262-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 68262-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 68262-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 68262-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FUS/TLS antibody (68262-1-Ig, Clone: 1B4F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 68262-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FUS/TLS antibody (68262-1-Ig, Clone: 1B4F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse cerebellum using 68262-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse cerebellum tissue using FUS/TLS antibody (68262-1-Ig, Clone: 1B4F8 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-3), Calbindin-D28k antibody (14479-1-AP, green).

• 

  IF Staining of rat cerebellum using 68262-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat cerebellum tissue using FUS/TLS antibody (68262-1-Ig, Clone: 1B4F8 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-3), Calbindin-D28k antibody (14479-1-AP, green).

• 

  FC experiment of HEK-293T using 68262-1-Ig

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human FUS/TLS (68262-1-Ig, Clone:1B4F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules Neuro-2a, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IHC: tissu cérébral de rat, tissu cérébral de souris, tissu de cervelet de souris, tissu de côlon humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF-Fro: tissu de cervelet de rat, tissu de cervelet de souris
• Résultats positifs en FC (Intra): cellules HEK-293T,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:5000-1:20000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below

Informations sur le produit

68262-1-Ig cible FUS/TLS dans les applications de WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FUS/TLS Protéine recombinante Ag2150
• Nom complet: fusion (involved in t(12;16) in malignant liposarcoma)
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 53 kDa, 68-75 kDa
• Numéro d’acquisition GenBank: BC026062
• Symbole du gène: FUS/TLS
• Identification du gène (NCBI): 2521
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)(PMID: 22640227). Multiple phosphorylation on the N terminus of FUS caused that FUS was detected 68-75 kDa (PMID:24899704).

Publications

Species	Application	Title
		Mol Cell; A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis; Authors - Kaiwen Bao
		Mol Cell; Aberrant phase separation drives membranous organelle remodeling and tumorigenesis; Authors - Xinyu Wang
human	WB	Arterioscler Thromb Vasc Biol; Exosomes From IgE-Stimulated Mast Cells Aggravate Asthma-Mediated Atherosclerosis Through circRNA CDR1as-Mediated Endothelial Cell Dysfunction in Mice; Authors - Hongqin Yang
human	WB	Clin Exp Med; FUS and METTL3 collaborate to regulate RNA maturation, preventing unfolded protein response and promoting gastric cancer progression; Authors - Dongtao Liu ; KD Validated
rat	WB	Nat Neurosci; Mitochondrial respiratory complex IV deficiency recapitulates amyotrophic lateral sclerosis; Authors - Man Cheng
mouse	WB	Cell; Microglial-derived C1q integrates into neuronal ribonucleoprotein complexes and impacts protein homeostasis in the aging brain; Authors - Nicole Scott-Hewitt

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Vikas (Verified Customer) (04-08-2025)

very good. Highly recomended

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK-293T

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