Anticorps Polyclonal de lapin anti-FUS/TLS

• Phare
• Validé par KD/KO

FUS/TLS Polyclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite®555 Fluorescent Dye

N° de cat : CL555-11570

Synonymes

FUS, 75 kDa DNA pairing protein, 75 kDa DNA-pairing protein, ALS, ALS6

---

Galerie de données de validation

• 

  IF Staining of MCF-7 using CL555-11570

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®555 FUS/TLS antibody (CL555-11570) at dilution of 1:200.

• 

  IF Staining of HeLa using CL555-11570

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 FUS/TLS antibody (CL555-11570) at dilution of 1:200, CL488-phalloidin (green).

• 

  FC experiment of MCF-7 using CL555-11570

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human FUS/TLS (CL555-11570) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Applications testées

• Résultats positifs en IF/ICC: cellules MCF-7, cellules HeLa
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-11570 cible FUS/TLS dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FUS/TLS Protéine recombinante Ag2150
• Nom complet: fusion (involved in t(12;16) in malignant liposarcoma)
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 68-75 kDa
• Numéro d’acquisition GenBank: BC026062
• Symbole du gène: FUS/TLS
• Identification du gène (NCBI): 2521
• Conjugaison: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)[PMID: 22640227]. This antibody is a rabbit polyclonal antibody raised against an internal region of human FUS.

{{ptg:RelatedPrimaryAntibodies}}