Anticorps Monoclonal anti-FUT8

• Phare
• Validé par KD/KO

FUT8 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(15)

CloneNo.

2F7H2

N° de cat : 66118-1-Ig

Synonymes

2F7H2, Alpha-(1,6)-fucosyltransferase, alpha1 6FucT, Alpha1-6FucT, EC:2.4.1.68

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Galerie de données de validation

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  WB analysis of MDA-MB-231 using 66118-1-Ig

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 66118-1-Ig (FUT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66118-1-Ig

  WB result of FUT8 antibody (66118-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FUT8 transfected MCF-7 cells.

• 

  WB analysis of MDA-MB-453s using 66118-1-Ig

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 66118-1-Ig (FUT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66118-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66118-1-Ig (FUT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 66118-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66118-1-Ig (FUT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 66118-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66118-1-Ig (FUT8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66118-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66118-1-Ig (FUT8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 66118-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66118-1-Ig (FUT8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 66118-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66118-1-Ig (FUT8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66118-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66118-1-Ig (FUT8 Antibody) at dilution of 1:50 under 40x lens.

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  IHC staining of human lung cancer using 66118-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66118-1-Ig (FUT8 Antibody) at dilution of 1:50 under 10x lens.

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  IHC staining of human lung cancer using 66118-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66118-1-Ig (FUT8 Antibody) at dilution of 1:50 under 40x lens.

• 

  IHC staining of human breast cancer using 66118-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66118-1-Ig (FUT8 Antibody) at dilution of 1:50 under 10x lens.

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Applications testées

• Résultats positifs en WB: cellules MDA-MB-231, cellules Caco-2, cellules MCF-7, cellules MDA-MB-453s
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du poumon humain, tissu de cancer du sein humain, tissu de cervelet de souris, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Informations sur le produit

66118-1-Ig cible FUT8 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FUT8 Protéine recombinante Ag18769
• Nom complet: fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC025385
• Symbole du gène: FUT8
• Identification du gène (NCBI): 2530
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fucosyltransferase 8 (FUT8) is GDP-fucose-glycoprotein fucosyltransferase and is localized in Golgi apparatus. FUT8 is involved in protein glycosylation by catalyzing the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. FUT8 may be involved in human aging by regulating protein N-glycosylation and in the molecular pathogenesis of human pulmonary emphysema (PE).

Publications

Species	Application	Title
human	WB	Cancer Cell; A Systems Biology Approach Identifies FUT8 as a Driver of Melanoma Metastasis.; Authors - Praveen Agrawal ; KD Validated
human	IF	Nat Commun; EGFR core fucosylation, induced by hepatitis C virus, promotes TRIM40-mediated-RIG-I ubiquitination and suppresses interferon-I antiviral defenses; Authors - Qiu Pan
human	WB	Am J Hum Genet; Biallelic Mutations in FUT8 Cause a Congenital Disorder of Glycosylation with Defective Fucosylation.; Authors - Bobby G Ng
human	IHC	Mol Cell Proteomics; Glycomic Profiling Highlights Increased Fucosylation in Pseudomyxoma Peritonei.; Authors - Lilli Saarinen
human	WB,IHC	Am J Cancer Res; Fucosyltransferase 8 modulates receptor tyrosine kinase activation and temozolomide resistance in glioblastoma cells.; Authors - Kuo-Chen Wei ; KD Validated
human	IHC	Mol Cell Proteomics; Glycomic Profiling Highlights Increased Fucosylation in Pseudomyxoma Peritonei; Authors - Lilli Saarinen

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