Anticorps Monoclonal anti-FUT8

• Phare
• Validé par KD/KO

FUT8 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2F7H2

N° de cat : 66118-1-PBS

Synonymes

2F7H2, Alpha-(1,6)-fucosyltransferase, alpha1 6FucT, Alpha1-6FucT, EC:2.4.1.68

---

Galerie de données de validation

• 

  WB analysis of MDA-MB-231 using 66118-1-Ig (same clone as 66118-1-PBS)

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 66118-1-Ig (FUT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66118-1-Ig (same clone as 66118-1-PBS)

  WB result of FUT8 antibody (66118-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FUT8 transfected MCF-7 cells. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MDA-MB-453s using 66118-1-Ig (same clone as 66118-1-PBS)

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 66118-1-Ig (FUT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66118-1-Ig (same clone as 66118-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66118-1-Ig (FUT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Caco-2 using 66118-1-Ig (same clone as 66118-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66118-1-Ig (FUT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66118-1-Ig (same clone as 66118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66118-1-Ig (FUT8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66118-1-Ig (same clone as 66118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66118-1-Ig (FUT8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 66118-1-Ig (same clone as 66118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66118-1-Ig (FUT8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 66118-1-Ig (same clone as 66118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66118-1-Ig (FUT8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66118-1-Ig (same clone as 66118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66118-1-Ig (FUT8 Antibody) at dilution of 1:50 under 40x lens. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66118-1-Ig (same clone as 66118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66118-1-Ig (FUT8 Antibody) at dilution of 1:50 under 10x lens. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66118-1-Ig (same clone as 66118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66118-1-Ig (FUT8 Antibody) at dilution of 1:50 under 40x lens. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66118-1-Ig (same clone as 66118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66118-1-Ig (FUT8 Antibody) at dilution of 1:50 under 10x lens. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66118-1-PBS cible FUT8 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FUT8 Protéine recombinante Ag18769
• Nom complet: fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC025385
• Symbole du gène: FUT8
• Identification du gène (NCBI): 2530
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Fucosyltransferase 8 (FUT8) is GDP-fucose-glycoprotein fucosyltransferase and is localized in Golgi apparatus. FUT8 is involved in protein glycosylation by catalyzing the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. FUT8 may be involved in human aging by regulating protein N-glycosylation and in the molecular pathogenesis of human pulmonary emphysema (PE).

{{ptg:RelatedPrimaryAntibodies}}