Anticorps Monoclonal anti-FUT9

• Phare
• Validé par KD/KO

FUT9 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

5D4G6

N° de cat : 60230-1-Ig

Synonymes

Alpha (1,3) fucosyltransferase, Fuc TIX, Fucosyltransferase 9, Fucosyltransferase IX, FucT IX, FUT9

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Galerie de données de validation

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  WB analysis of pig brain using 60230-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60230-1-Ig (FUT9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 60230-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60230-1-Ig (FUT9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 60230-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60230-1-Ig (FUT9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 60230-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60230-1-Ig (FUT9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 60230-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 60230-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60230-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60230-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 60230-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:50.

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  IHC staining of human stomach using 60230-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:50.

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  IHC staining of human stomach using 60230-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach using 60230-1-Ig(FUT9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach using 60230-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach using 60230-1-Ig(FUT9 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of human breast cancer using 60230-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using FUT9 antibody (60230-1-Ig, Clone: 5D4G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain fœtal
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du sein humain, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du sein humain,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

60230-1-Ig cible FUT9 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FUT9 Protéine recombinante Ag8298
• Nom complet: fucosyltransferase 9 (alpha (1,3) fucosyltransferase)
• Masse moléculaire calculée: 359 aa, 42 kDa
• Poids moléculaire observé: 44-46 kDa
• Numéro d’acquisition GenBank: BC036101
• Symbole du gène: FUT9
• Identification du gène (NCBI): 10690
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FUT9 is the main enzyme responsible for the synthesis of Lewis X (Lex) and catalyzes the last step in the biosynthesis of Lewis X antigen by addition of a fucose residue to precursor glycan structures. FUT9 is expressed in stomach, kidney, brain, and in leukocytes.

Publications

Species	Application	Title
mouse	WB	Cancers (Basel); FUT9-Driven Programming of Colon Cancer Cells towards a Stem Cell-Like State.; Authors - Athanasios Blanas ; KO Validated
human	WB,IHC	Acta Biochim Biophys Sin (Shanghai); ELF4 contributes to esophageal squamous cell carcinoma growth and metastasis by augmenting cancer stemness via FUT9; Authors - Aiping Xu ; KD Validated

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