Anticorps Polyclonal de lapin anti-Phospholemman/FXYD1

Phospholemman/FXYD1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

27

N° de cat : 13721-1-AP

Synonymes

FXYD1, Phospholemman, Phospholemman/FXYD1, PLM

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Galerie de données de validation

• 

  WB analysis of human skeletal muscle using 13721-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13721-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 13721-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 13721-1-AP (Phospholemman/FXYD1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 13721-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13721-1-AP (Phospholemman/FXYD1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 13721-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13721-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 13721-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 13721-1-AP

  IP Result of anti-Phospholemman/FXYD1 (IP:13721-1-AP, 4ug; Detection:13721-1-AP 1:500) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of mouse heart using 13721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 13721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 13721-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 13721-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 13721-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13721-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique humain, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral humain, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris, tissu rénal de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cardiaque humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1500
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 22 publications below
• IF: See 1 publications below

Informations sur le produit

13721-1-AP cible Phospholemman/FXYD1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, Lapin, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Phospholemman/FXYD1 Protéine recombinante Ag4669
• Nom complet: FXYD domain containing ion transport regulator 1
• Masse moléculaire calculée: 92 aa, 10 kDa
• Poids moléculaire observé: 10-15 kDa
• Numéro d’acquisition GenBank: BC032800
• Symbole du gène: FXYD1
• Identification du gène (NCBI): 5348
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FXYD1, also named as PLM and Phospholemman, belongs to the FXYD family. FXYD1 induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. It may have a functional role in muscle contraction. FXYD1 is a partner protein and regulator of the Na+,K+-ATPase (Na+,K+-pump). It may play a role in the acute regulation of the Na+,K+-ATPase response to exercise. (PMID: 20595385, 21653224)

Publications

Species	Application	Title
mouse	WB,IF	Sci Rep; Over-expression of microRNA-1 causes arrhythmia by disturbing intracellular trafficking system.; Authors - Xiaomin Su
rat	WB	J Gen Physiol; Distinct α2 Na,K-ATPase membrane pools are differently involved in early skeletal muscle remodeling during disuse.; Authors - Violetta V Kravtsova
rat	WB	J Biol Chem; The nicotinic acetylcholine receptor and the Na,K-ATPase alpha2 isoform interact to regulate membrane electrogenesis in skeletal muscle.; Authors - Heiny Judith A JA
rat	WB	J Physiol; Fatigue-induced change in T-system excitability and its major cause in rat fast-twitch skeletal muscle in vivo.; Authors - Daiki Watanabe
human	WB	J Physiol; Cycling with blood flow restriction improves performance and muscle K+ regulation and alters the effect of anti-oxidant infusion in humans.; Authors - Danny Christiansen
human,mouse,rat	WB	Am J Physiol Endocrinol Metab; Effect of exercise and training on phospholemman phosphorylation in human skeletal muscle.; Authors - Benziane Boubacar B

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