Anticorps Monoclonal anti-FXYD1

FXYD1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C10G9

N° de cat : 67150-1-PBS

Synonymes

FXYD1, Phospholemman, PLM

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Galerie de données de validation

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  WB analysis of human heart using 67150-1-Ig (same clone as 67150-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 67150-1-Ig (FXYD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67150-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skeletal muscle using 67150-1-Ig (same clone as 67150-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67150-1-Ig (FXYD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67150-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig heart using 67150-1-Ig (same clone as 67150-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67150-1-Ig (FXYD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67150-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 67150-1-Ig (same clone as 67150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67150-1-Ig (FXYD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67150-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 67150-1-Ig (same clone as 67150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67150-1-Ig (FXYD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67150-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 67150-1-Ig (same clone as 67150-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using FXYD1 antibody (67150-1-Ig, Clone: 2C10G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67150-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 67150-1-Ig (same clone as 67150-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using FXYD1 antibody (67150-1-Ig, Clone: 2C10G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67150-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67150-1-PBS cible FXYD1 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FXYD1 Protéine recombinante Ag25307
• Nom complet: FXYD domain containing ion transport regulator 1
• Masse moléculaire calculée: 92 aa, 10 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC032800
• Symbole du gène: FXYD1
• Identification du gène (NCBI): 5348
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FXYD1, also named as PLM and Phospholemman, belongs to the FXYD family. FXYD1 induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. It may have a functional role in muscle contraction. FXYD1 is a partner protein and regulator of the Na+,K+-ATPase (Na+,K+-pump). It may play a role in the acute regulation of the Na+,K+-ATPase response to exercise. (PMID: 20595385, 21653224)

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