Anticorps Monoclonal anti-FYN

• Phare
• Validé par KD/KO

FYN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(19)

CloneNo.

1G4B1

Avis (1)

N° de cat : 66606-1-Ig

Synonymes

1G4B1, EC:2.7.10.2, p59 Fyn, p59-Fyn, Proto oncogene c Fyn

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Galerie de données de validation

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  WB analysis using 66606-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66606-1-Ig

  WB result of FYN antibody (66606-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FYN transfected HEK-293 cells.

• 

  WB analysis using 66606-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66606-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 66606-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66606-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66606-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66606-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66606-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66606-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66606-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66606-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66606-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66606-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66606-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66606-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66606-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66606-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66606-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Neuro-2a using 66606-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using FYN antibody (66606-1-Ig, Clone: 1G4B1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 66606-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug FYN Monoclonal antibody (66606-1-Ig, Clone:1G4B1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules 4T1, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, cellules PC-12, cellules Ramos
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules Neuro-2a,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:30000
• Immunohistochimie (IHC): IHC : 1:2000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 15 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

66606-1-Ig cible FYN dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FYN Protéine recombinante Ag11127
• Nom complet: FYN oncogene related to SRC, FGR, YES
• Masse moléculaire calculée: 537aa,61 kDa; 482aa,55 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC032496
• Symbole du gène: FYN
• Identification du gène (NCBI): 2534
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FYN, also named p59-Fyn and SLK, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. FYN is implicated in the control of cell growth. It plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. FYN is required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. FYN blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC.

Publications

Species	Application	Title
mouse	WB	EBioMedicine; Obesity induces preadipocyte CD36 expression promoting inflammation via the disruption of lysosomal calcium homeostasis and lysosome function.; Authors - Xiaoxiao Luo
mouse,human	IHC	EBioMedicine; Biallelic mutations in LAMA5 disrupts a skeletal noncanonical focal adhesion pathway and produces a distinct bent bone dysplasia.; Authors - Maya Barad
human		Inflammation; hPMSCs Regulate the Level of TNF-α and IL-10 in Th1 Cells and Improve Hepatic Injury in a GVHD Mouse Model via CD73/ADO/Fyn/Nrf2 Axis; Authors - Hengchao Zhang
human	WB	Biochem Pharmacol; PIKE-A promotes glioblastoma growth by driving PPP flux through increasing G6PD expression mediated by phosphorylation of STAT3.; Authors - Mingming Sun
human	IHC	Front Oncol; A four oxidative stress gene prognostic model and integrated immunity-analysis in pancreatic adenocarcinoma; Authors - Hao Wang
mouse	WB	ACS Omega; Catalpol Promotes Osseointegration of Titanium Implants under Conditions of Type 2 Diabetes via AKT/GSK3β/FYN Pathway-Mediated NRF2 Activation; Authors - Yongzhi Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

X (Verified Customer) (07-11-2022)

A reliable antibody in WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

FYN Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:66606-1-Ig)

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