Anticorps Monoclonal anti-FYN

• Phare
• Validé par KD/KO

FYN Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G4B1

N° de cat : 66606-1-PBS

Synonymes

1G4B1, EC:2.7.10.2, p59 Fyn, p59-Fyn, Proto oncogene c Fyn

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Galerie de données de validation

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  WB analysis using 66606-1-Ig (same clone as 66606-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66606-1-Ig (same clone as 66606-1-PBS)

  WB result of FYN antibody (66606-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FYN transfected HEK-293 cells. This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66606-1-Ig (same clone as 66606-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66606-1-Ig (same clone as 66606-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

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  WB analysis of Ramos using 66606-1-Ig (same clone as 66606-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66606-1-Ig (same clone as 66606-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66606-1-Ig (same clone as 66606-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66606-1-Ig (same clone as 66606-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66606-1-Ig (same clone as 66606-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66606-1-Ig (same clone as 66606-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66606-1-Ig (same clone as 66606-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66606-1-Ig (same clone as 66606-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66606-1-Ig (same clone as 66606-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66606-1-Ig (same clone as 66606-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66606-1-Ig (same clone as 66606-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66606-1-Ig (same clone as 66606-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66606-1-Ig (same clone as 66606-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66606-1-Ig (same clone as 66606-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66606-1-Ig (same clone as 66606-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

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  IF Staining of Neuro-2a using 66606-1-Ig (same clone as 66606-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using FYN antibody (66606-1-Ig, Clone: 1G4B1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66606-1-Ig (same clone as 66606-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug FYN Monoclonal antibody (66606-1-Ig, Clone:1G4B1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66606-1-PBS cible FYN dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FYN Protéine recombinante Ag11127
• Nom complet: FYN oncogene related to SRC, FGR, YES
• Masse moléculaire calculée: 537aa,61 kDa; 482aa,55 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC032496
• Symbole du gène: FYN
• Identification du gène (NCBI): 2534
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FYN, also named p59-Fyn and SLK, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. FYN is implicated in the control of cell growth. It plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. FYN is required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. FYN blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC.

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