Anticorps Polyclonal de lapin anti-Frizzled 2

• Phare
• Validé par KD/KO

Frizzled 2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(13)

N° de cat : 24272-1-AP

Synonymes

FZD2, Frizzled-2, Fz 2, Fz-2, FzE2

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Galerie de données de validation

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  WB analysis using 24272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24272-1-AP (Frizzled 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 24272-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 24272-1-AP (Frizzled 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24272-1-AP (Frizzled 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 24272-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 24272-1-AP (Frizzled 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 24272-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24272-1-AP (Frizzled 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 24272-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24272-1-AP (Frizzled 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HEK-293, tissu de côlon de souris, tissu rénal de rat
• Résultats positifs en IHC: tissu de côlon de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IF: See 1 publications below

Informations sur le produit

24272-1-AP cible Frizzled 2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Frizzled 2 Protéine recombinante Ag18003
• Nom complet: frizzled homolog 2 (Drosophila)
• Masse moléculaire calculée: 565 aa, 64 kDa
• Poids moléculaire observé: 64-70 kDa
• Numéro d’acquisition GenBank: BC113402
• Symbole du gène: Frizzled 2
• Identification du gène (NCBI): 2535
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Frizzled 2(FZD2) also known as FzE2, which belongs to the G-protein coupled receptor Fz/Smo family. FZD2 is an important receptor in the Wnt pathway, which is highly expressed in malignant tumors and helps regulate multiple tumor behaviors. Its expression level is related to prognosis. Moreover, high level of FZD2 had significant correlation with poor prognosis in Breast cancer (BC) patients(PMID: 33832493).

Publications

Species	Application	Title
mouse	WB	Biol Res; Electroacupuncture promotes neurogenesis in the dentate gyrus and improves pattern separation in an early Alzheimer's disease mouse model; Authors - Yanyi Ding
human	WB	Front Microbiol; Expression and (Lacking) Internalization of the Cell Surface Receptors of Clostridioides difficile Toxin B.; Authors - Dennis Schöttelndreier
rat	WB	Pharm Biol; Yiguanjian decoction inhibits macrophage M1 polarization and attenuates hepatic fibrosis induced by CCl4/2-AAF.; Authors - Ying Xu
mouse	WB	Evid Based Complement Alternat Med; Intervention Mechanism of Hunag-Lian Jie-Du Decoction on Canonical Wnt/β-Catenin Signaling Pathway in Psoriasis Mouse Model.; Authors - Xuesong Yang
human	WB	Cell Death Discov; Cholesterol activates the Wnt/PCP-YAP signaling in SOAT1-targeted treatment of colon cancer.; Authors - Huanji Xu ; KD Validated
human	WB	Genes Dis; Depletion of VPS35 attenuates metastasis of hepatocellular carcinoma by restraining the Wnt/PCP signaling pathway.; Authors - Yi Liu

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