Anticorps Polyclonal de lapin anti-Frizzled 2

Frizzled 2 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 24272-1-AP

Synonymes

Frizzled 2, Fz 2, FZD2, FzE2, hFz2

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Galerie de données de validation

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  WB analysis using 24272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24272-1-AP (Frizzled 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 24272-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 24272-1-AP (Frizzled 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24272-1-AP (Frizzled 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 24272-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 24272-1-AP (Frizzled 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 24272-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Frizzled 2 antibody (24272-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HEK-293, tissu de côlon de souris, tissu rénal de rat
• Résultats positifs en IF: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below

Informations sur le produit

24272-1-AP cible Frizzled 2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Frizzled 2 Protéine recombinante Ag18003
• Nom complet: frizzled homolog 2 (Drosophila)
• Masse moléculaire calculée: 565 aa, 64 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC113402
• Symbole du gène: FZD2
• Identification du gène (NCBI): 2535
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Front Microbiol; Expression and (Lacking) Internalization of the Cell Surface Receptors of Clostridioides difficile Toxin B.; Authors - Dennis Schöttelndreier
rat	WB	Pharm Biol; Yiguanjian decoction inhibits macrophage M1 polarization and attenuates hepatic fibrosis induced by CCl4/2-AAF.; Authors - Ying Xu
mouse	WB	Evid Based Complement Alternat Med; Intervention Mechanism of Hunag-Lian Jie-Du Decoction on Canonical Wnt/β-Catenin Signaling Pathway in Psoriasis Mouse Model.; Authors - Xuesong Yang
human	WB	Cell Death Discov; Cholesterol activates the Wnt/PCP-YAP signaling in SOAT1-targeted treatment of colon cancer.; Authors - Huanji Xu ; KD Validated
human	WB	Genes Dis; Depletion of VPS35 attenuates metastasis of hepatocellular carcinoma by restraining the Wnt/PCP signaling pathway.; Authors - Yi Liu
human	WB	Theranostics; Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia.; Authors - Wenjia Liu