Anticorps Polyclonal de lapin anti-Frizzled 5

Frizzled 5 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (2)

N° de cat : 21519-1-AP

Synonymes

C2orf31, Frizzled 5, Fz 5, FZD5, FzE5, HFZ5

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Galerie de données de validation

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  WB analysis of COLO 320 using 21519-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21519-1-AP (FZD5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 21519-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21519-1-AP (Frizzled 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 21519-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21519-1-AP (FZD5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 21519-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21519-1-AP (Frizzled 5 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 21519-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21519-1-AP (Frizzled 5 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 21519-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 21519-1-AP (FZD5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 21519-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 21519-1-AP (FZD5 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 21519-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Frizzled 5 antibody (21519-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 21519-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Frizzled 5 antibody (21519-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules COLO 320, cellules Jurkat, cellules PC-12, cellules PC-3, tissu de côlon de rat, tissu de côlon de souris, tissu d'intestin grêle de souris
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

21519-1-AP cible Frizzled 5 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Frizzled 5 Protéine recombinante Ag16082
• Nom complet: frizzled homolog 5 (Drosophila)
• Masse moléculaire calculée: 585 aa, 65 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC114346
• Symbole du gène: FZD5
• Identification du gène (NCBI): 7855
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fzd5, is the receptor for the Wnt5A ligand. Fzd5 modulates cell fate and cell behavior during vertebrate development. It regulates hematopoiesis by the antagonism of the canonical Wnt pathway, resulting in a pool of quiescent hematopoietic stem cells, and regulates hematopoietic stem cell function in a paracrine fashion through several distinct mechanisms including modulating canonical Wnt signaling. It acts through noncanonical Wnt signaling to promote angiogenesis. It is also involved in control cell orientation, polarity, and directional movement in response to positional cues from chemokine gradients.

Publications

Species	Application	Title
human	WB	Cell Death Discov; Cholesterol activates the Wnt/PCP-YAP signaling in SOAT1-targeted treatment of colon cancer.; Authors - Huanji Xu ; KD Validated
human	WB	Theranostics; Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia.; Authors - Wenjia Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Susanne (Verified Customer) (04-17-2023)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Fibroblasts

Frizzled 5 Antibody Immunofluorescence validation (1:100 dilution) in Fibroblasts (Cat no:21519-1-AP)

FH

Louisiane (Verified Customer) (08-11-2022)

PFA fixed tissues (small intestine sections) were incubated over the weekend at 4C with primary antibody and overnight at 4C with secondary antibody. No signal detected in the small intestine.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 to 100