Anticorps Polyclonal de lapin anti-Frizzled 7

• Phare
• Validé par KD/KO

Frizzled 7 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(16)

Avis (2)

N° de cat : 16974-1-AP

Synonymes

Frizzled 7, Fz 7, FZD7, FzE3, hFz7

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Galerie de données de validation

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  WB analysis of mouse skeletal muscle using 16974-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16974-1-AP (Frizzled 7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 16974-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16974-1-AP (Frizzled 7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16974-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16974-1-AP (Frizzled 7 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 16974-1-AP

  IP result of anti-Frizzled 7 (IP:16974-1-AP, 4ug; Detection:16974-1-AP 1:600) with mouse skeletal muscle tissue lysate 2200ug.

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  IHC staining of human small intestine using 16974-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16974-1-AP (Frizzled 7 antibody) at dilution of 1:800 (under 10x lens).

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  IHC staining of human small intestine using 16974-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16974-1-AP (Frizzled 7 antibody) at dilution of 1:800 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu cardiaque de souris, tissu rénal de souris
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu d'intestin grêle humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

16974-1-AP cible Frizzled 7 dans les applications de WB, IHC, IF, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Frizzled 7 Protéine recombinante Ag9446
• Nom complet: frizzled homolog 7 (Drosophila)
• Masse moléculaire calculée: 574 aa, 64 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC015915
• Symbole du gène: Frizzled 7
• Identification du gène (NCBI): 8324
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Frizzled class receptor 7 (Frizzled 7, also known as FZD7) is a member of the superfamily of G protein-coupled receptor proteins that act as receptors in the Wnt signaling pathway. Frizzled 7 plays a role in development, stem cell renewal, and differentiation and has been implicated in the progression of various cancers.

What is the molecular weight of Frizzled 7? Is Frizzled 7 post-translationally modified?

The molecular size of Frizzled 7 is ~63 kDa, which represents the mature N-glycosylated species (PMID: 25274627). Frizzled 7 can form homodimers and heterodimers with other Frizzled family members (PMID: 14688793).

What is the subcellular localization of Frizzled 7?

Frizzled 7 is a seven-pass transmembrane protein that is present at the plasma membrane. In endothelial cells, it predominantly locates to cell-cell junctions (PMID: 24866384). Frizzled 7 has a cleavage site for calpain-1 protease in its C-terminus that regulates its activity and plasma membrane turnover (PMID: 17716656).

What is the tissue expression pattern of Frizzled 7?

Frizzled 7 is expressed at high levels in adult skeletal muscles and in fetal kidneys, but is also present in fetal lung, adult heart, brain, and placenta (PMID: 9813155). Frizzled 7 expression is increased in various cancer types, including melanoma, hepatocellular carcinoma, lung, esophageal, gastric, and colon cancers.

Publications

Species	Application	Title
human,mouse	WB,IHC	Adv Mater; m6 A Reader YTHDF1-Targeting Engineered Small Extracellular Vesicles for Gastric Cancer Therapy via Epigenetic and Immune Regulation; Authors - Qing You
human	CoIP	Cell Death Differ; Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway.; Authors - Regina Cheuk-Lam Lo
human	WB	Mol Syst Biol; Systematic protein-protein interaction mapping for clinically relevant human GPCRs.; Authors - Kate Sokolina
human	WB	Cancer Res; YTHDF1 promotes gastric carcinogenesis by controlling translation of FZD7.; Authors - Jingnan Pi
mouse	WB,IF,RIP	Arch Med Res; RNA-binding Protein QKI Inhibits Osteogenic Differentiation Via Suppressing Wnt Pathway; Authors - Zhao Yan
human	IF	Apoptosis; YTHDF1-mediated m6A modification promotes cisplatin resistance in ovarian cancer via the FZD7/Wnt/β-catenin pathway; Authors - Jintian Miao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Susanne (Verified Customer) (04-17-2023)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Fibroblasts

Frizzled 7 Antibody Immunofluorescence validation (1:500 dilution) in Fibroblasts (Cat no:16974-1-AP)

FH

Louisiane (Verified Customer) (08-11-2022)

Tissues (small intestine sections) were incubated with primary antibody over the weekend at 4C and with secondary antibody overnight at 4C. The signal looks good

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 to 100

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