• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-Frizzled 7

Frizzled 7 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

N° de cat : 16974-1-AP

Synonymes

Frizzled 7, Fz 7, FZD7, FzE3, hFz7



Applications testées

Résultats positifs en WBtissu de muscle squelettique de souris, tissu cardiaque de souris, tissu rénal de souris
Résultats positifs en IPtissu de muscle squelettique de souris
Résultats positifs en IHCtissu d'intestin grêle humain,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

16974-1-AP cible Frizzled 7 dans les applications de WB, RIP, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Frizzled 7 Protéine recombinante Ag9446
Nom complet frizzled homolog 7 (Drosophila)
Masse moléculaire calculée 574 aa, 64 kDa
Poids moléculaire observé 64 kDa
Numéro d’acquisition GenBankBC015915
Symbole du gène FZD7
Identification du gène (NCBI) 8324
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Frizzled class receptor 7 (Frizzled 7, also known as FZD7) is a member of the superfamily of G protein-coupled receptor proteins that act as receptors in the Wnt signaling pathway. Frizzled 7 plays a role in development, stem cell renewal, and differentiation and has been implicated in the progression of various cancers.

What is the molecular weight of Frizzled 7? Is Frizzled 7 post-translationally modified?

The molecular size of Frizzled 7 is ~63 kDa, which represents the mature N-glycosylated species (PMID: 25274627). Frizzled 7 can form homodimers and heterodimers with other Frizzled family members (PMID: 14688793).

What is the subcellular localization of Frizzled 7?

Frizzled 7 is a seven-pass transmembrane protein that is present at the plasma membrane. In endothelial cells, it predominantly locates to cell-cell junctions (PMID: 24866384). Frizzled 7 has a cleavage site for calpain-1 protease in its C-terminus that regulates its activity and plasma membrane turnover (PMID: 17716656).

What is the tissue expression pattern of Frizzled 7?

Frizzled 7 is expressed at high levels in adult skeletal muscles and in fetal kidneys, but is also present in fetal lung, adult heart, brain, and placenta (PMID: 9813155). Frizzled 7 expression is increased in various cancer types, including melanoma, hepatocellular carcinoma, lung, esophageal, gastric, and colon cancers.


Protocole

Product Specific Protocols
WB protocol for Frizzled 7 antibody 16974-1-APDownload protocol
IHC protocol for Frizzled 7 antibody 16974-1-APDownload protocol
IP protocol for Frizzled 7 antibody 16974-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Adv Mater

m6 A Reader YTHDF1-Targeting Engineered Small Extracellular Vesicles for Gastric Cancer Therapy via Epigenetic and Immune Regulation

Authors - Qing You
humanCoIP

Cell Death Differ

Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway.

Authors - Regina Cheuk-Lam Lo
humanWB

Mol Syst Biol

Systematic protein-protein interaction mapping for clinically relevant human GPCRs.

Authors - Kate Sokolina
humanWB

Cancer Res

YTHDF1 promotes gastric carcinogenesis by controlling translation of FZD7.

Authors - Jingnan Pi
mouseWB,IF,RIP

Arch Med Res

RNA-binding Protein QKI Inhibits Osteogenic Differentiation Via Suppressing Wnt Pathway

Authors - Zhao Yan
humanWB

Front Microbiol

Expression and (Lacking) Internalization of the Cell Surface Receptors of Clostridioides difficile Toxin B.

Authors - Dennis Schöttelndreier

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Susanne (Verified Customer) (04-17-2023)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Fibroblasts
Frizzled 7 Antibody Immunofluorescence validation (1:500 dilution) in Fibroblasts (Cat no:16974-1-AP)
FH

Louisiane (Verified Customer) (08-11-2022)

Tissues (small intestine sections) were incubated with primary antibody over the weekend at 4C and with secondary antibody overnight at 4C. The signal looks good

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1 to 100