Anticorps Recombinant de lapin anti-Factor IX/PTC

Factor IX/PTC Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241742G4

N° de cat : 84533-4-PBS

Synonymes

F9, Factor IX, Christmas factor, coagulation factor IX, Coagulation factor IXa heavy chain

---

Galerie de données de validation

• 

  WB analysis of human plasma using 84533-4-RR (same clone as 84533-4-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 84533-4-RR (Coagulation factor IX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84533-4-PBS in a different storage buffer formulation.

• 

  IHC staining of Human Hepatocellular cancer using 84533-4-RR (same clone as 84533-4-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84533-4-RR (Factor IX/PTC antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84533-4-PBS in a different storage buffer formulation.

---

Informations sur le produit

84533-4-PBS cible Factor IX/PTC dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Factor IX/PTC Protéine recombinante Eg1045
• Nom complet: coagulation factor IX
• Masse moléculaire calculée: 52kDa
• Poids moléculaire observé: 72 kDa
• Numéro d’acquisition GenBank: NM_000133.4
• Symbole du gène: F9
• Identification du gène (NCBI): 2158
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The F9 gene encodes coagulation factor IX, which circulates as an inactive zymogen until proteolytic release of its activation peptide allows it to assume the conformation of an active serine protease. Its role in the blood coagulation cascade is to activate factor X (F10) through interactions with calcium, membrane phospholipids, and factor VIII (F8). Factor IX and factor X both consist of 2 polypeptide chains referred to as the L (light) and H (heavy) chains. The H chain bears a structural resemblance to the polypeptide chain of the pancreatic serine protease trypsin (PRSS1). The L chain is covalently linked to the H chain by a single disulfide bond. Native Factor IX MW is around 72kDa(PMID:827443; 12496253), and it can be cleaved into different fragments.

{{ptg:RelatedPrimaryAntibodies}}