Anticorps Monoclonal anti-Factor X

Factor X Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3D3F11

N° de cat : 66753-1-PBS

Synonymes

F10, 3D3F11, Activated factor Xa heavy chain, coagulation factor X, EC:3.4.21.6

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Galerie de données de validation

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  WB analysis of human plasma using 66753-1-Ig (same clone as 66753-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66753-1-Ig (Factor X antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66753-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66753-1-Ig (same clone as 66753-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66753-1-Ig (Factor X antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66753-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66753-1-Ig (same clone as 66753-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66753-1-Ig (Factor X antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66753-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver using 66753-1-Ig (same clone as 66753-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Factor X antibody (66753-1-Ig, Clone: 3D3F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66753-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66753-1-PBS cible Factor X dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Factor X Protéine recombinante Ag11664
• Nom complet: coagulation factor X
• Masse moléculaire calculée: 488 aa, 55 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC046125
• Symbole du gène: F10
• Identification du gène (NCBI): 2159
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Coagulation factor X (F10) is a vitamin K-dependent glycoprotein that participates in the middle phase of the blood coagulation cascade (PMID: 1931959; 1313796). It is synthesized as a single-chain precursor in the liver where it undergoes a number of covalent processing steps before secretion into the blood as a two-chain molecule linked by a disulfide bond (PMID: 1313796). The light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. Factor X is activated into factor Xa, by both the extrinsic and intrinsic pathway. The activated factor then converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.

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