Anticorps Monoclonal anti-Factor XIIIa

Factor XIIIa Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D2B2

N° de cat : 66325-1-PBS

Synonymes

F13A1, Protein-glutamine gamma-glutamyltransferase A chain, F13A, EC:2.3.2.13, Coagulation factor XIIIa

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Galerie de données de validation

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  WB analysis using 66325-1-Ig (same clone as 66325-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

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  WB analysis of human peripheral blood platelets using 66325-1-Ig (same clone as 66325-1-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit large intestine using 66325-1-Ig (same clone as 66325-1-PBS)

  rabbit large intestine tissue was subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 66325-1-Ig (same clone as 66325-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIA Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

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  WB analysis of rat colon using 66325-1-Ig (same clone as 66325-1-PBS)

  rat colon tissue was subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66325-1-Ig (same clone as 66325-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66325-1-Ig (Factor XIIIA antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66325-1-Ig (same clone as 66325-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66325-1-Ig (Factor XIIIA antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

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  FC experiment of U-937 using 66325-1-Ig (same clone as 66325-1-PBS)

  1x10^6 U-937 cells were intracellularly stained with 0.25 ug Factor XIIIa Monoclonal antibody (66325-1-Ig, Clone:2D2B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66325-1-PBS cible Factor XIIIa dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Factor XIIIa Protéine recombinante Ag11105
• Nom complet: coagulation factor XIII, A1 polypeptide
• Masse moléculaire calculée: 732 aa, 83 kDa
• Poids moléculaire observé: 75-80 kDa
• Numéro d’acquisition GenBank: BC027963
• Symbole du gène: Factor XIIIa
• Identification du gène (NCBI): 2162
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Factor XIIIa is a blood proenzyme that catalyzes intermolecular cross-linking of fibrinogen and is involved in clotting cascade. It has been identified in various tissues including placenta, uterus, and prostate. Anti- Factor XIIIa has been found to be useful in differentiating between dermatofibroma (90% positive) and desmoplastic malignant melanoma (negative). Factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor , and meningioma.

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