Anticorps Polyclonal de lapin anti-Fas/CD95

• Phare
• Validé par KD/KO

Fas/CD95 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13098-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of Jurkat using 13098-1-AP (same clone as 13098-1-PBS)

  WB result of FAS/CD95 antibody (13098-1-AP, 1:1000) with si-control and si-FAS transfected Jurkat cells. This data was developed using the same antibody clone with 13098-1-PBS in a different storage buffer formulation.

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  WB analysis using 13098-1-AP (same clone as 13098-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13098-1-AP (FAS/CD95 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13098-1-PBS in a different storage buffer formulation.

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  WB analysis using 13098-1-AP (same clone as 13098-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13098-1-AP (FAS/CD95 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13098-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 13098-1-AP (same clone as 13098-1-PBS)

  IP result of anti-FAS/CD95 (IP:13098-1-AP, 3ug; Detection:13098-1-AP 1:500) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 13098-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13098-1-PBS cible Fas/CD95 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Fas/CD95 Protéine recombinante Ag3718
• Nom complet: Fas (TNF receptor superfamily, member 6)
• Masse moléculaire calculée: 35-38 kDa
• Poids moléculaire observé: 38-45 kDa
• Numéro d’acquisition GenBank: BC012479
• Symbole du gène: Fas/CD95
• Identification du gène (NCBI): 355
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Fas (CD95/APO-1) is a transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. It can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The molecular mass of native Fas is 38 kDa, the high molecular weight form (40-55 kDa) of Fas is due to glycosylation.

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