Anticorps Polyclonal de lapin anti-Fas/CD95

Fas/CD95 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30601-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 30601-1-AP (same clone as 30601-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30601-1-AP (Fas/CD95 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30601-1-PBS in a different storage buffer formulation.

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  WB analysis using 30601-1-AP (same clone as 30601-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30601-1-AP (Fas/CD95 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30601-1-PBS in a different storage buffer formulation.

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  IF Staining of Raji using 30601-1-AP (same clone as 30601-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using Fas/CD95 antibody (30601-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30601-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30601-1-PBS cible Fas/CD95 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Fas/CD95 Protéine recombinante Ag30426
• Nom complet: Fas (TNF receptor superfamily member 6)
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 30-40 kDa
• Numéro d’acquisition GenBank: NM_007987
• Symbole du gène: Fas/CD95
• Identification du gène (NCBI): 14102
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.

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