Anticorps Recombinant de lapin anti-Fas/CD95

Fas/CD95 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

240340E11

N° de cat : 98034-1-RR

Synonymes

FAS, APT1, Apoptosis-mediating surface antigen FAS, Apo-1 antigen, APO-1

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Galerie de données de validation

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  FC experiment of human PBMCs using 98034-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Fas/CD95 Rabbit Recombinant Antibody (98034-1-RR, Clone: 240340E11) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD45RO (UCHL1) (APC-65150, Clone: UCHL1). Cells were not fixed.

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  FC experiment of human PBMCs using 98034-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Fas/CD95 Rabbit RecAb (98034-1-RR, Clone: 240340E11) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with FITC Plus Anti-Human CD45RO. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98034-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Fas/CD95 Rabbit RecAb (98034-1-RR, Clone:240340E11) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98034-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Fas/CD95 Rabbit Recombinant Antibody (98034-1-RR, Clone: 240340E11) (red), or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98034-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98034-1-RR against Human Fas/CD95 were performed. The affinity constant is 1.97 nM.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98034-1-RR cible Fas/CD95 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: Fas (TNF receptor superfamily, member 6)
• Masse moléculaire calculée: 38 kDa
• Numéro d’acquisition GenBank: NM_000043.6
• Symbole du gène: Fas/CD95
• Identification du gène (NCBI): 355
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.

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