Anticorps Monoclonal anti-Fascin

• Phare
• Validé par KD/KO

Fascin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B5G3

N° de cat : 66321-1-PBS

Synonymes

FSCN1, Fascin 1,Fascin, FAN1, 55 kDa actin-bundling protein, 55 kDa actin bundling protein

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 66321-1-Ig (same clone as 66321-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66321-1-Ig (Fascin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 66321-1-Ig (same clone as 66321-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66321-1-Ig (Fascin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66321-1-Ig (same clone as 66321-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66321-1-Ig (Fascin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66321-1-Ig (Fascin Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66321-1-Ig (Fascin Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66321-1-Ig (Fascin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66321-1-Ig (same clone as 66321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66321-1-Ig (Fascin Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66321-1-Ig (same clone as 66321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66321-1-Ig (Fascin Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66321-1-Ig (same clone as 66321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66321-1-Ig (Fascin Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66321-1-Ig (same clone as 66321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66321-1-Ig (Fascin Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66321-1-Ig (Fascin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Fascin antibody (66321-1-Ig, Clone: 1B5G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 66321-1-Ig (same clone as 66321-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 66321-1-Ig(Fascin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 66321-1-Ig (same clone as 66321-1-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Fascin Monoclonal antibody (66321-1-Ig, Clone:1B5G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66321-1-PBS cible Fascin dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Fascin Protéine recombinante Ag6074
• Nom complet: fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC000521
• Symbole du gène: Fascin
• Identification du gène (NCBI): 6624
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.

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