Anticorps Polyclonal de lapin anti-Ferritin heavy chain

• Phare
• Validé par KD/KO

Ferritin heavy chain Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11682-1-PBS

Synonymes

Ferritin, FTH1, Cell proliferation-inducing gene 15 protein, EC:1.16.3.1, Ferritin heavy chain, N-terminally processed

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Galerie de données de validation

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  WB analysis using 11682-1-AP (same clone as 11682-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11682-1-AP (same clone as 11682-1-PBS)

  WB result of Ferritin heavy chain antibody (11682-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ferritin heavy chain transfected HeLa cells. This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

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  WB analysis using 11682-1-AP (same clone as 11682-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11682-1-AP (same clone as 11682-1-PBS)

  IP result of anti-Ferritin heavy chain (IP:11682-1-AP, 4ug; Detection:11682-1-AP 1:3000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 11682-1-AP (same clone as 11682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 11682-1-AP (same clone as 11682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 11682-1-AP (same clone as 11682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 11682-1-AP (same clone as 11682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11682-1-AP (same clone as 11682-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ferritin heavy chain antibody (11682-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11682-1-AP (same clone as 11682-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ferritin heavy chain antibody (11682-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11682-1-PBS cible Ferritin heavy chain dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Ferritin heavy chain Protéine recombinante Ag2290
• Nom complet: ferritin, heavy polypeptide 1
• Masse moléculaire calculée: 183 aa, 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC000857
• Symbole du gène: FTH1
• Identification du gène (NCBI): 2495
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.

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