Anticorps Monoclonal anti-Fetuin-A/AHSG

Fetuin-A/AHSG Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F6B9

N° de cat : 66094-1-PBS

Synonymes

AHSG, Fetuin-A, Alpha 2 Z globulin, alpha 2 HS glycoprotein, AHS

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Galerie de données de validation

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  WB analysis of HepG2 using 66094-1-Ig (same clone as 66094-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66094-1-Ig (Fetuin-A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

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  WB analysis of human plasma using 66094-1-Ig (same clone as 66094-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 66094-1-Ig (Fetuin-A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66094-1-Ig (same clone as 66094-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66094-1-Ig (Fetuin-A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 66094-1-Ig (same clone as 66094-1-PBS)

  IP result of anti-Fetuin-A (IP:66094-1-Ig, 3ug; Detection:66094-1-Ig 1:3000) with HepG2 cells lysate 6000ug. This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66094-1-Ig (same clone as 66094-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 66094-1-Ig(Fetuin-A antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66094-1-Ig (same clone as 66094-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 66094-1-Ig(Fetuin-A antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66094-1-Ig (same clone as 66094-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66094-1-Ig(Fetuin-A antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66094-1-Ig (same clone as 66094-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66094-1-Ig(Fetuin-A antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66094-1-Ig (same clone as 66094-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Fetuin-A antibody (66094-1-Ig, Clone: 1F6B9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66094-1-Ig (same clone as 66094-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Fetuin-A/AHSG Monoclonal antibody (66094-1-Ig, Clone:1F6B9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66094-1-PBS cible Fetuin-A/AHSG dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Fetuin-A/AHSG Protéine recombinante Ag10073
• Nom complet: alpha-2-HS-glycoprotein
• Masse moléculaire calculée: 367 aa, 39 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC048198
• Symbole du gène: Fetuin-A
• Identification du gène (NCBI): 197
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Fetuin-A (alpha-2-HS-glycoprotein, AHSG), a liver borne plasma protein, contributes to the prevention of soft tissue calcification, modulates inflammation, reduces INS sensitivity and fosters weight gain following high fat diet or ageing. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues.

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