Anticorps Monoclonal anti-Fibulin 5

• Phare
• Validé par KD/KO

Fibulin 5 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2A5C8

N° de cat : 60081-1-PBS

Synonymes

FBLN5, 2A5C8, Dance, Developmental arteries and neural crest EGF-like protein, EVEC

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Galerie de données de validation

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  WB analysis of MCF-7 using 60081-1-Ig (same clone as 60081-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 60081-1-Ig (same clone as 60081-1-PBS)

  WB result of Fibulin 5 antibody (60081-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Fibulin 5 transfected MCF-7 cells. This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

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  WB analysis of pig heart using 60081-1-Ig (same clone as 60081-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 60081-1-Ig (same clone as 60081-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 60081-1-Ig (same clone as 60081-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 60081-1-Ig (same clone as 60081-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 60081-1-Ig (same clone as 60081-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed A549 cells using 60081-1-Ig(Fibulin 5 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60081-1-PBS cible Fibulin 5 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Fibulin 5 Protéine recombinante Ag2859
• Nom complet: fibulin 5
• Masse moléculaire calculée: 448 aa, 50 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC022280
• Symbole du gène: Fibulin 5
• Identification du gène (NCBI): 10516
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Fibulin-5, also known as EVEC and DANCE, is a extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies.

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