Anticorps Polyclonal de lapin anti-Ficolin 2

Ficolin 2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 32442-1-PBS

Synonymes

FCN2, FCNL, Ficolin B, Ficolin beta, Hucolin

---

Galerie de données de validation

• 

  WB analysis using 32442-1-AP (same clone as 32442-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 32442-1-AP (FCN2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32442-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 32442-1-AP (same clone as 32442-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32442-1-AP (Ficolin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32442-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 32442-1-AP (same clone as 32442-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32442-1-AP (Ficolin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32442-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

32442-1-PBS cible Ficolin 2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Ficolin 2 Protéine recombinante Ag37525
• Nom complet: ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin)
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34-35 kDa
• Numéro d’acquisition GenBank: NM_004108
• Symbole du gène: FCN2
• Identification du gène (NCBI): 2220
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ficolins are a group of oligomeric lectins with subunits consisting of both collagen-like and fibrinogen-like domains (PMID: 20375620). They are innate pattern recognition receptors and play integral roles in the immune response. In humans, there are three ficolins termed M-, L-, and H-ficolin (also referred to as ficolin-1, -2, and -3) whereas rodents only possess ficolin-A and -B, which are the orthologues of human L- and M-ficolin, respectively (PMID: 30868077). Ficolin 2 recognizes pathogen-associated pattern molecules (PAMPs) on the surface of pathogenic microorganisms, activates the complement lectin pathway, and binds to mannose-binding lectin-associated serine proteases (MASPs) to exert immune defense function (PMID: 21315829).

{{ptg:RelatedPrimaryAntibodies}}