Anticorps Monoclonal anti-DYKDDDDK Tag
DYKDDDDK Tag Monoclonal Antibody for WB
Hôte / Isotype
Mouse / IgG2b
Réactivité testée
Protéine recombinante et plus (1)
Applications
WB, CoIP, ELISA
Conjugaison
HRP
CloneNo.
8H6A10
N° de cat : HRP-66008
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | Protéine recombinante |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:100000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 24 publications below |
| ELISA | See 1 publications below |
| CoIP | See 1 publications below |
Informations sur le produit
HRP-66008 cible DYKDDDDK Tag dans les applications de WB, CoIP, ELISA et montre une réactivité avec des échantillons Protéine recombinante
| Réactivité | Protéine recombinante |
| Réactivité citée | souris |
| Hôte / Isotype | Mouse / IgG2b |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | Peptide |
| Nom complet | DYKDDDDK Tag |
| Symbole du gène | |
| Identification du gène (NCBI) | |
| Conjugaison | HRP |
| Forme | Liquide |
| Méthode de purification | Purification par protéine A |
| Tampon de stockage | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
| Conditions de stockage | Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
DYKDDDDK tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDYKDDDDK. The structure of DYKDDDDK tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with DYKDDDDK tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 1E7B4, which is different from any other vendors. This antibody is HRP conjugated DYKDDDDK Tag antibody. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP DYKDDDDK Tag antibody HRP-66008 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cancer Exosomal circ_0006896 promotes AML progression via interaction with HDAC1 and restriction of antitumor immunity | ||
Ann Rheum Dis Lupus and inflammatory bowel disease share a common set of microbiome features distinct from other autoimmune disorders | ||
Nat Commun A lung-selective delivery of mRNA encoding broadly neutralizing antibody against SARS-CoV-2 infection | ||
Nat Commun FAM222A encodes a protein which accumulates in plaques in Alzheimer's disease. | ||
PLoS Pathog Vibrio splendidus infection promotes circRNA-FGL1-regulated coelomocyte apoptosis via competitive binding to Myc with the deubiquitinase OTUB1 in Apostichopus japonicus | ||
Int Immunopharmacol ZAP facilitates NLRP3 inflammasome activation via promoting the oligomerization of NLRP3 |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Chunxu (Verified Customer) (01-31-2025) | Significant facility the process of WB and this antibody results great specifity
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