Anticorps Monoclonal anti-DDDDK tag

DDDDK tag Monoclonal Antibody for ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

2C3F4

N° de cat : 66008-1-Ig

Synonymes

DDDDK tag


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  • Immunoprecipitation (IP) experiment of Transfected HEK-293 cells using DDDDK tag Monoclonal antibody (Binds to FLAG® tag  (66008-1-Ig)

    IP experiment of Transfected HEK-293 using 66008-1-Ig

    IP Result of anti-Flag tag (IP:66008-1-Ig, 3ug; Detection:66008-1-Ig 1:10000) with Transfected HEK-293 cells lysate 300ug.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using DDDDK tag Monoclonal antibody (Binds to FLAG® tag  (66008-1-Ig)

    IF Staining of Transfected HEK-293 using 66008-1-Ig

    Immunofluorescent analysis of Transfected HEK-293 cells using 66008-1-Ig (Flag tag antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

"DDDDK tag Antibodies" Comparison

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Applications testées

Dilution recommandée

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

66008-1-Ig cible DDDDK tag dans les applications de WB, RIP, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain, souris
Hôte / Isotype Mouse / IgG2b
Clonalité Monoclonal
Type Anticorps
Immunogène Peptide
Nom complet DDDDK tag
Symbole du gène
Identification du gène (NCBI)
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDDDK tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDDDDK. The structure of DDDDK tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, the DDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with DDDDK tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 2C3F4, which is different from any other vendors.

Protocole

Product Specific Protocols
IF protocol for DDDDK tag antibody 66008-1-IgDownload protocol
IP protocol for DDDDK tag antibody 66008-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Nat Microbiol

Ephrin receptor A2 is an epithelial cell receptor for Epstein-Barr virus entry.

Authors - Hua Zhang
WB

Cell Metab

Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.

Authors - Xiao Wang
WB

Neuron

UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model.

Authors - Kejing Zhang
WB

Plant Cell

Structural Insight into DNA Recognition by CCT/NF-YB/YC Complexes in Plant Photoperiodic Flowering.

Authors - Cuicui Shen
humanWB

Cell Death Dis

The E3 ligase TRIM26 suppresses ferroptosis through catalyzing K63-linked ubiquitination of GPX4 in glioma

Authors - Zhangjie Wang
WB

Mol Ther

Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.

Authors - Benfu Zhong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Chun (Verified Customer) (07-03-2019)

This antibody is excellent

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK293 and HeLa cells, and primary rat cortical neurons