Anticorps Monoclonal anti-DYKDDDDK tag

DYKDDDDK tag Monoclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Protéine recombinante et plus (7)

Applications

WB, RIP, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

CloneNo.

8H6A10

N° de cat : 66008-4-Ig

Synonymes

DDDDK, DDDDK tag, DYKDDDDK, DYKDDDDK tag, Flag, Flag tag


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  • Western Blot (WB) analysis of various lysates using DYKDDDDK tag Monoclonal antibody (Binds to FLAG® t (66008-4-Ig)

    WB analysis using 66008-4-Ig

    HEK-293 and different loading amount of DYKDDDDK tagged TDP-43 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDK tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using DYKDDDDK tag Monoclonal antibody (Binds to FLAG® t (66008-4-Ig)

    WB analysis using 66008-4-Ig

    HEK-293 and DYKDDDDK-TDP43 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDK tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading

  • Western Blot (WB) analysis of various lysates using DYKDDDDK tag Monoclonal antibody (Binds to FLAG® t (66008-4-Ig)

    WB analysis using 66008-4-Ig

    HEK-293 and DYKDDDDK-PCSK9 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDKtag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading control.

  • Immunoprecipitation (IP) experiment of Transfected HEK-293 cells using DYKDDDDK tag Monoclonal antibody (Binds to FLAG® t (66008-4-Ig)

    IP experiment of Transfected HEK-293 using 66008-4-Ig

    IP result of anti-DYKDDDDK tag(IP:66008-4-Ig, 5 ug; Detection:80010-1-RR 1:10000) with Transfected HEK-293T cells lysate 250 ug.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using DYKDDDDK tag Monoclonal antibody (Binds to FLAG® t (66008-4-Ig)

    IF Staining of Transfected HEK-293 using 66008-4-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed DYKDDDDK-TDP43 Transfected HEK-293 cells using DYKDDDDK tag antibody (66008-4-Ig, Clone: 8H6A10 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

"DYKDDDDK tag Antibodies" Comparison

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Applications testées

Résultats positifs en WBTransfected HEK-293 cells
Résultats positifs en IPcellules HEK-293 transfectées,
Résultats positifs en IFcellules HEK-293 transfectées,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

66008-4-Ig cible DYKDDDDK tag dans les applications de WB, RIP, IP, IHC, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Protéine recombinante

Réactivité Protéine recombinante
Réactivité citéebovin, canin, Humain, poisson-zèbre, porc, poulet, souris
Hôte / Isotype Mouse / IgG2b
Clonalité Monoclonal
Type Anticorps
Immunogène Peptide
Nom complet DYKDDDDK tag
Symbole du gène
Identification du gène (NCBI)
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Protocole

Product Specific Protocols
WB protocol for DYKDDDDK tag antibody 66008-4-IgDownload protocol
IF protocol for DYKDDDDK tag antibody 66008-4-IgDownload protocol
IP protocol for DYKDDDDK tag antibody 66008-4-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ChIP

Blood

Functional, structural and molecular characterizations of leukemogenic driver MEF2D-HNRNPUL1 fusion.

Authors - Ming Zhang
WB

Nat Commun

UV-B irradiation-activated E3 ligase GmILPA1 modulates gibberellin catabolism to increase plant height in soybean

Authors - Jiaqi Sun
IP

Nat Commun

Homodimer-mediated phosphorylation of C/EBPα-p42 S16 modulates acute myeloid leukaemia differentiation through liquid-liquid phase separation

Authors - Dongmei Wang
humanWB

Adv Sci (Weinh)

Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy

Authors - Qian Gou
humanWB,IP

Cell Death Differ

Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration

Authors - Huaizhen Liang
WB,IF,IP

Cell Death Differ

Deubiquitinase OTUD6A in macrophages promotes intestinal inflammation and colitis via deubiquitination of NLRP3

Authors - Xin Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (12-12-2022)

worked very well

  • Applications: Western Blot, Immunofluorescence
FH

Chun (Verified Customer) (06-19-2022)

This antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1,000
FH

Céline (Verified Customer) (03-29-2022)

I have compared with anti-Flag M2 from Sigma in an IP experiment and I found the M2 antibody better.

  • Applications: Immunoprecipitation
  • Primary Antibody Dilution: 1/100
  • Cell Tissue Type: Hela cells