Anticorps Polyclonal de lapin anti-Flightless I

Flightless I Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 27729-1-AP

Synonymes

Protein flightless-1 homolog, FLIL, FLII, Flightless-1

---

Galerie de données de validation

• 

  WB analysis using 27729-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27729-1-AP (Flightless I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27729-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27729-1-AP (Flightless I antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 27729-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27729-1-AP (Flightless I antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 27729-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27729-1-AP (Flightless I antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 27729-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Flightless I antibody (27729-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HeLa, cellules T-47D, cellules THP-1
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

27729-1-AP cible Flightless I dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Flightless I Protéine recombinante Ag26865
• Nom complet: flightless I homolog (Drosophila)
• Masse moléculaire calculée: 1269 aa, 145 kDa
• Poids moléculaire observé: 145-150 kDa
• Numéro d’acquisition GenBank: BC025300
• Symbole du gène: Flightless I
• Identification du gène (NCBI): 2314
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Flightless I (FliI) is the most evolutionarily conserved member of the gelsolin superfamily of proteins which are key regulators of actin filament assembly and turnover. FliI comprises an N-terminal leucine-rich repeat (LRR) domain which is not present in other gelsolin family members, and the LRR domain may enable interactions between FliI and other molecules involved in signal transduction, thereby spatially integrating signaling and actin remodeling functions. This protein was originally found in Drosophila and participates in the embryonic development, while mammalian FliI protein was involved in the regulation of wound repair,skin barrier development. Studies recently demonstrated that FliI protein associated with colorectal cancer, hepatocellular and prostate cancer (PMID:30091651; 28498392).

{{ptg:RelatedPrimaryAntibodies}}