Anticorps Monoclonal anti-Flightless I

Flightless I Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2F9C8

N° de cat : 67039-1-PBS

Synonymes

FLI, Fli1, Flightless I, FLII, FLIL, Protein flightless 1 homolog

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Galerie de données de validation

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  WB analysis using 67039-1-Ig (same clone as 67039-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67039-1-Ig (FLII antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67039-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67039-1-Ig (same clone as 67039-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67039-1-Ig (FLII antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67039-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67039-1-Ig (same clone as 67039-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67039-1-Ig (FLII antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67039-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67039-1-Ig (same clone as 67039-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67039-1-Ig (FLII antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67039-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67039-1-PBS cible Flightless I dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Flightless I Protéine recombinante Ag26865
• Nom complet: flightless I homolog (Drosophila)
• Masse moléculaire calculée: 1269 aa, 145 kDa
• Poids moléculaire observé: 145-150 kDa
• Numéro d’acquisition GenBank: BC025300
• Symbole du gène: Flightless I
• Identification du gène (NCBI): 2314
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Flightless I (FliI) is the most evolutionarily conserved member of the gelsolin superfamily of proteins which are key regulators of actin filament assembly and turnover. FliI comprises an N-terminal leucine-rich repeat (LRR) domain which is not present in other gelsolin family members, and the LRR domain may enable interactions between FliI and other molecules involved in signal transduction, thereby spatially integrating signaling and actin remodeling functions. This protein was originally found in Drosophila and participates in the embryonic development, while mammalian FliI protein was involved in the regulation of wound repair,skin barrier development. Studies recently demonstrated that FliI protein associated with colorectal cancer, hepatocellular and prostate cancer (PMID:30091651; 28498392).

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