Anticorps Recombinant de lapin anti-Flotillin 1

Flotillin 1 Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

240582G6

N° de cat : 83598-4-PBS

Synonymes

FLOT1, 240582G6, Flotillin-1

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Galerie de données de validation

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  WB analysis using 83598-4-RR (same clone as 83598-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83598-4-RR (Flotillin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83598-4-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 83598-4-RR (same clone as 83598-4-PBS)

  IP result of anti-Flotillin 1 (IP:83598-4-RR, 4ug; Detection:83598-4-RR 1:3000) with mouse brain tissue lysate 2400 ug. This data was developed using the same antibody clone with 83598-4-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83598-4-RR (same clone as 83598-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83598-4-RR (Flotillin 1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83598-4-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83598-4-RR (same clone as 83598-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83598-4-RR (Flotillin 1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83598-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83598-4-RR (same clone as 83598-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Flotillin 1 antibody (83598-4-RR, Clone: 240582G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83598-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83598-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83598-4-RR against Human Flotillin 1 were performed. The affinity constant is 30.5 nM.

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Informations sur le produit

83598-4-PBS cible Flotillin 1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Flotillin 1 Protéine recombinante Ag7902
• Nom complet: flotillin 1
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC001146
• Symbole du gène: Flotillin 1
• Identification du gène (NCBI): 10211
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins commonly used as lipid raft markers. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.

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