Anticorps Polyclonal de lapin anti-Flotillin 2

Flotillin 2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(8)

Avis (1)

N° de cat : 28208-1-AP

Synonymes

FLOT2, Flotillin-2, ECS 1, ECS1, Epidermal surface antigen

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Galerie de données de validation

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  WB analysis using 28208-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28208-1-AP (Flotillin 2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 28208-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28208-1-AP (Flotillin 2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 28208-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Flotillin 2 antibody (28208-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules A549, cellules HEK-293, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

28208-1-AP cible Flotillin 2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Flotillin 2 Protéine recombinante Ag28308
• Nom complet: flotillin 2
• Masse moléculaire calculée: 379 aa, 42 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC017292
• Symbole du gène: Flotillin 2
• Identification du gène (NCBI): 2319
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.

Publications

Species	Application	Title
human	WB,IF	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
human	WB	J Exp Clin Cancer Res; RNA-binding protein CCDC137 activates AKT signaling and promotes hepatocellular carcinoma through a novel non-canonical role of DGCR8 in mRNA localization; Authors - Shuang Tao
human	WB	Int J Biol Sci; miR-34a-5p functions as a tumor suppressor in head and neck squamous cell cancer progression by targeting Flotillin-2.; Authors - Xiang Li
human	IHC	Int J Biol Sci; TBL1X and Flot2 form a positive feedback loop to promote metastasis in nasopharyngeal carcinoma.; Authors - Hongjuan Xu
human	WB	Oncol Rep; PLCD3, a flotillin2-interacting protein, is involved in proliferation, migration and invasion of nasopharyngeal carcinoma cells.; Authors - Weidong Liu
human	WB	Alzheimers Dement; Amyloid futures in the expanding pathology of brain aging and dementia; Authors - Max A Thorwald

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Guorong (Verified Customer) (03-22-2022)

Very weak bands with strong background were detected at 48 kDa

• Applications: Western Blot
• Primary Antibody Dilution: 1:1500
• Cell Tissue Type: ARPE-19

Flotillin 2 Antibody Western Blot validation (1:1500 dilution) in ARPE-19 (Cat no:28208-1-AP)

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