Anticorps Polyclonal de lapin anti-Flotillin 2

Flotillin 2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28208-1-PBS

Synonymes

FLOT2, Flotillin-2, ECS 1, ECS1, Epidermal surface antigen

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Galerie de données de validation

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  WB analysis using 28208-1-AP (same clone as 28208-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28208-1-AP (Flotillin 2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28208-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 28208-1-AP (same clone as 28208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28208-1-AP (Flotillin 2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28208-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 28208-1-AP (same clone as 28208-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Flotillin 2 antibody (28208-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28208-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28208-1-PBS cible Flotillin 2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Flotillin 2 Protéine recombinante Ag28308
• Nom complet: flotillin 2
• Masse moléculaire calculée: 379 aa, 42 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC017292
• Symbole du gène: Flotillin 2
• Identification du gène (NCBI): 2319
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.

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