Anticorps Monoclonal anti-Flotillin 2

Flotillin 2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E11G12

N° de cat : 66881-1-PBS

Synonymes

FLOT2, Flotillin-2, 1E11G12, ECS 1, ECS1

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Galerie de données de validation

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  WB analysis using 66881-1-Ig (same clone as 66881-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

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  WB analysis using 66881-1-Ig (same clone as 66881-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66881-1-Ig (same clone as 66881-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66881-1-Ig (same clone as 66881-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66881-1-Ig (same clone as 66881-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66881-1-Ig (same clone as 66881-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Flotillin 2 antibody (66881-1-Ig, Clone: 1E11G12 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66881-1-Ig (same clone as 66881-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Flotillin 2 Monoclonal antibody (66881-1-Ig, Clone:1E11G12) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66881-1-PBS cible Flotillin 2 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Flotillin 2 Protéine recombinante Ag28308
• Nom complet: flotillin 2
• Masse moléculaire calculée: 379 aa, 42 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC017292
• Symbole du gène: Flotillin 2
• Identification du gène (NCBI): 2319
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.

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