Anticorps Monoclonal anti-Foxp3

Foxp3 Monoclonal Antibody for IHC, FC (Intra)

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

souris

Applications

IHC, IF, FC (Intra)

Conjugaison

Non conjugué

Publications(9)

CloneNo.

3G3

N° de cat : 65089-1-Ig

Synonymes

Foxp3, JM2, scurfin, sf, 3G3, forkhead box P3

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Galerie de données de validation

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  IHC staining of mouse spleen using 65089-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 65089-1-Ig (Foxp3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 65089-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 65089-1-Ig (Foxp3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 65089-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 65089-1-Ig (Foxp3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of C57BL/c mouse splenocytes using 65089-1-Ig

  1x10^6 C57BL/c mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse Foxp3 (3G3) (65089-1-Ig, Clone: 3G3) or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21), FITC anti-Mouse IgG1 Antibody, and CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IHC: tissu splénique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): splénocytes de souris
• Résultats positifs en cytométrie: splénocytes de souris

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below
• IF: See 6 publications below

Informations sur le produit

65089-1-Ig cible Foxp3 dans les applications de IHC, IF, FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: forkhead box P3
• Numéro d’acquisition GenBank: BC132333
• Symbole du gène: Foxp3
• Identification du gène (NCBI): 20371
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

Foxp3 (forkhead box P3), also named as IPEX, JM2 and scurfin, is a member of the forkhead transcription factor family and is essential for normal immune homeostasis (PMID: 11138001; 20429413). Foxp3 is specifically expressed in CD4+ CD25+ regulatory T cells and is crucial for their development and suppressive activity (PMID: 12612578; 12612581). In scurfy mouse, disruption of Foxp3 results in the fatal lymphoproliferative disorder attributed to the lack of an immune-suppressive component in the immune system (PMID: 25683611; 11138001). Similar to mice, genetic defects in human Foxp3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome (PMID: 11137993).

Publications

Species	Application	Title
mouse	IHC	Thorac Cancer; Adiponectin-expressing Treg cells in experimental thymic tumor model; Authors - Yuki Hanamatsu
mouse	IF	Adv Sci (Weinh); Targeting L-Selectin Lymphocytes to Deliver Immunosuppressive Drug in Lymph Nodes for Durable Multiple Sclerosis Treatment; Authors - Yipeng Zhao
mouse	IF	Thorac Cancer; Adiponectin-expressing Treg-containing T cell fraction inhibits tumor growth in orthotopically implanted triple-negative breast cancer; Authors - Wakana Chikaishi
		Cell Rep; FBXO38 mediates FGL1 ubiquitination and degradation to enhance cancer immunity and suppress inflammation; Authors - Tongguan Tian
mouse	IF	Cell Commun Signal; Tumorous IRE1α facilitates CD8+T cells-dependent anti-tumor immunity and improves immunotherapy efficacy in melanoma; Authors - Yuqi Yang
mouse	IHC	J Ethnopharmacol; The extract from Quzhou Aurantii Fructus attenuates cough variant asthma through inhibiting the TRPV1/Ca2+/NFAT/TSLP pathway and ferroptosis via TRPV1 mediation in ovalbumin-induced mice; Authors - Meizi Tian

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